Lengeler K, Kothe E
Department of Biology, Molecular Genetics, Philipps-University, Marburg, FRG.
FEMS Microbiol Lett. 1994 Jun 1;119(1-2):243-7. doi: 10.1111/j.1574-6968.1994.tb06895.x.
The basis of the auxotrophic ura1 phenotype in Schizophyllum commune has been investigated. Two point mutations causing changes in conserved amino acid positions 62 (from lysine to glutamate) and 79 (from leucine to phenylalanine) most likely are the cause for the observed phenotype, whereas the overall gene structure was unchanged. Since reversion rates in this locus are extremely low, a single point mutation could not be expected to be the cause for the mutation. Besides the two point mutations expected to be induced by UV mutagenesis, the two alleles investigated from independently isolated strains differ by approximately 7% in nucleic acid sequence and about 3% in amino acid sequence, indicating a distant relationship between the strains used.
对裂褶菌中营养缺陷型ura1表型的基础进行了研究。两个导致保守氨基酸位置62（从赖氨酸变为谷氨酸）和79（从亮氨酸变为苯丙氨酸）发生变化的点突变很可能是观察到的表型的原因，而总体基因结构未发生改变。由于该位点的回复突变率极低，因此不能预期单个点突变是该突变的原因。除了预期由紫外线诱变诱导的两个点突变外，从独立分离的菌株中研究的两个等位基因在核酸序列上相差约7%，在氨基酸序列上相差约3%，这表明所用菌株之间存在较远的亲缘关系。
