Zbikowska Halina Malgorzata, Szejk Magdalena, Saluk Joanna, Pawlaczyk-Graja Izabela, Gancarz Roman, Olejnik Alicja Klaudia
Department of General Biochemistry, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska 141/143, 90-236 Lodz, Poland.
Department of General Biochemistry, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska 141/143, 90-236 Lodz, Poland.
Int J Biol Macromol. 2016 May;86:329-37. doi: 10.1016/j.ijbiomac.2016.01.090. Epub 2016 Mar 17.
Polyphenolic-polysaccharide macromolecular, water-soluble glycoconjugates, isolated from the selected medicinal plants of Rosaceae/Asteraceae family: from leaves of Fragaria vesca L., Rubus plicatus Whe. et N. E., and from flowering parts of Sanguisorba officinalis L., and Erigeron canadensis L., were investigated for their ability to protect proteins and lipids of human plasma against γ-radiation-induced oxidative damage. Treatment of plasma with plant conjugates (6, 30, 150 μg/ml) prior exposure to 100 Gy radiation resulted in a significant inhibition of lipid peroxidation, evaluated by TBARS levels; conjugates isolated from E. canadensis and R. plicatus and a reference flavonoid quercetin showed similar high potential (approx. 70% inhibition, at 6 μg/ml). The conjugates prevented radiation-induced oxidation of protein thiols and significantly improved plasma total antioxidant capacity, estimated with Ellman's reagent and ABTS(.+) assay, respectively. The results demonstrate by the first time a significant radioprotective capability of the polyphenolic-polysaccharide conjugates isolated from E. canadensis, R. plicatus, S. officinalis and to the less extent from F. vesca. The abilities of these substances to inhibit radiation-induced lipid peroxidation and thiol oxidation in plasma seems to be mediated, but not limited to ROS scavenging activity.
从蔷薇科/菊科选定药用植物中分离得到的多酚-多糖大分子水溶性糖缀合物：分别来自野草莓叶、皱叶悬钩子叶以及地榆花和加拿大飞蓬花，对其保护人血浆蛋白质和脂质免受γ辐射诱导的氧化损伤的能力进行了研究。在暴露于100 Gy辐射之前，用植物缀合物（6、30、150 μg/ml）处理血浆，通过硫代巴比妥酸反应物（TBARS）水平评估，结果显示脂质过氧化受到显著抑制；从加拿大飞蓬和皱叶悬钩子中分离得到的缀合物以及参考类黄酮槲皮素显示出相似的高潜力（在6 μg/ml时约70%抑制率）。这些缀合物可防止辐射诱导的蛋白质巯基氧化，并分别用埃尔曼试剂和ABTS（.+）测定法显著提高血浆总抗氧化能力。结果首次证明，从加拿大飞蓬、皱叶悬钩子、地榆中分离得到的多酚-多糖缀合物具有显著的辐射防护能力，从野草莓中分离得到的缀合物也有一定程度的辐射防护能力。这些物质抑制血浆中辐射诱导的脂质过氧化和巯基氧化的能力似乎是由活性氧清除活性介导的，但不仅限于此。
