Kazemi Sima, Saidijam Massoud, Hashemi Seyed Hamid, Karami Manoochehr, Vaisi-Raygani Asad, Alikhani Mohammad Yousef
a Department of Microbiology, Faculty of Medicine , Hamadan University of Medical Sciences , Hamadan , Iran.
b Research Center for Molecular Medicine, School of Medicine , Hamadan University of Medical Sciences , Hamadan , Iran.
Immunol Invest. 2016;45(2):107-15. doi: 10.3109/08820139.2015.1096285. Epub 2016 Feb 5.
It seems that polymorphism in the regulatory areas of cytokine genes affects the cytokine production capacity and may play a role in the development of infectious diseases. Interleukin-10 (IL-10) and Interleukin-6 (IL-6), which are cytokines of Th2, cause the macrophage become inactive and patient conditions get worse.
In this case-control study, 60 patients with brucellosis and 60 healthy participants were recruited. IL-10 genotyping at positions -1082 (G/A), -819 (C/T), and -592 (C/A) and IL-6 genotyping at position -174 (G/C) were analyzed by amplification refractory mutation system-polymerase chain reaction (ARMS-PCR) and restriction fragment length polymorphism-polymerase chain reaction (RFLP-PCR) methods. The levels of IL-10 and IL-6 were determined by a sandwich enzyme-linked immunosorbent assay in sera of study population.
The AA and CC genotypes of the IL-10 gene at positions -1082 G/A and -819 C/T were significantly more frequent in patients in comparison to controls, respectively. The AG genotype of the IL-10 gene at positions -1082 G/A was significantly more frequent in control groups than the patients. Serum levels of IL-10 and IL-6 were significantly more frequent in the patients than in the control groups.
Our study showed that the AA and CC genotypes at positions -1082 and -819 are very important, respectively. These results suggest that IL-10 (-1082 G/A) GG genotype may be considered as a risk factor for brucellosis, while the AG genotype might be a protective factor against the disease.
细胞因子基因调控区的多态性似乎会影响细胞因子的产生能力，并可能在传染病的发生发展中起作用。白细胞介素-10（IL-10）和白细胞介素-6（IL-6）是Th2细胞因子，可使巨噬细胞失活，从而使患者病情加重。
在这项病例对照研究中，招募了60例布鲁氏菌病患者和60名健康参与者。通过扩增阻滞突变系统-聚合酶链反应（ARMS-PCR）和限制性片段长度多态性-聚合酶链反应（RFLP-PCR）方法分析了-1082（G/A）、-819（C/T）和-592（C/A）位点的IL-10基因分型以及-174（G/C）位点的IL-6基因分型。通过夹心酶联免疫吸附测定法测定研究人群血清中IL-10和IL-6的水平。
与对照组相比，患者中IL-10基因-1082 G/A和-819 C/T位点的AA和CC基因型频率分别显著更高。对照组中IL-10基因-1082 G/A位点的AG基因型频率显著高于患者。患者血清中IL-10和IL-6的水平显著高于对照组。
我们的研究表明，-1082和-819位点的AA和CC基因型分别非常重要。这些结果表明，IL-10（-1082 G/A）GG基因型可能被视为布鲁氏菌病的危险因素，而AG基因型可能是预防该病的保护因素。
