Asaei Sadaf, Rasouli Manoochehr, Moravej Ali
Department of Immunology, Professor Alborzi Clinical Microbiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran, e-mail:
Iran J Immunol. 2013 Sep;10(3):158-66.
Increased levels of interleukin-8 (IL-8) and interleukin-6 (IL-6) in acute human brucellosis have been reported. Previous studies have shown that the production and level of IL-6 and IL-8 cytokines are associated with the polymorphism of the encoding genes.
To investigate the probable association between IL-6 (-174 C/G) and IL-8 (-251 A/T) gene polymorphisms and susceptibility/resistance to brucellosis.
The patient group included 196 patients suffering from Brucella infection and the control group consisted of 82 healthy animal husbandmen from the same geographical area. IL-8 (-251 A/C) and IL-6 (-174 C/G) gene polymorphisms were analyzed by PCR-RFLP and Allele Specific PCR (AS-PCR) respectively.
The frequency of -251 IL-8 AA genotype was significantly lower in the controls compared with that of the patients (p=0.0051), while the frequencies of other genotypes (AT and TT) and alleles (A and T) were not significantly different among the participants. No association was found between IL-6 (-174 C/G) polymorphism and brucellosis.
This study indicates that the IL-8 -251 AA genotype may be considered as a genetic susceptibility factor for brucellosis.
据报道，人类急性布鲁氏菌病患者体内白细胞介素-8（IL-8）和白细胞介素-6（IL-6）水平升高。此前研究表明，IL-6和IL-8细胞因子的产生及水平与编码基因的多态性相关。
探讨IL-6（-174 C/G）和IL-8（-251 A/T）基因多态性与布鲁氏菌病易感性/抗性之间可能存在的关联。
患者组包括196例布鲁氏菌感染患者，对照组由来自同一地理区域的82名健康牧民组成。分别采用聚合酶链反应-限制性片段长度多态性分析（PCR-RFLP）和等位基因特异性PCR（AS-PCR）分析IL-8（-251 A/C）和IL-6（-174 C/G）基因多态性。
对照组中-251 IL-8 AA基因型频率显著低于患者组（p = 0.0051），而其他基因型（AT和TT）及等位基因（A和T）频率在参与者之间无显著差异。未发现IL-6（-174 C/G）多态性与布鲁氏菌病之间存在关联。
本研究表明，IL-8 -251 AA基因型可能被视为布鲁氏菌病的遗传易感性因素。
