Lv Zheng Jun, Liu Yang, Miao Hui, Leng Zhi Qian, Guo Jian Hui, Liu Jing
Regenerative Medicine Center, the First Affiliated Hospital of Dalian Medical University, Dalian, 116011, People's Republic of China.
Queen Mary University of London, London, E1 4NS, United Kingdom.
J Biomed Mater Res B Appl Biomater. 2017 Jul;105(5):934-943. doi: 10.1002/jbm.b.33620. Epub 2016 Feb 5.
The repair of nerves remains a major challenge in neuron-regeneration. In this study, poly(lactic-co-glycolic acid)/multi-walled carbon nanotubes (PLGA/MWCNTs) nanofibrous scaffolds were fabricated by electrospinning method. The surface morphology, physical, and mechanical properties were characterized through scanning electron microscopy (SEM), transmission electron microscopy, and tensile tests, respectively. SEM analysis, Live/Dead staining, immunostaining assays were performed to evaluate neural cells growth. Blending PLGA with MWCNTs resulted in increase diameter and porosity of the scaffolds, and exhibited better mechanical properties. The results demonstrated that the scaffolds with higher MWCNTs concentration provided better survival for neural cells after 8 days of culture, especially for astrocytes growth. This could be useful in treating the disease like multiple sclerosis that causing central nervous system demyelination and axonal injury. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 934-943, 2017.
神经修复仍然是神经元再生中的一项重大挑战。在本研究中，通过静电纺丝法制备了聚乳酸-乙醇酸共聚物/多壁碳纳米管（PLGA/MWCNTs）纳米纤维支架。分别通过扫描电子显微镜（SEM）、透射电子显微镜和拉伸试验对其表面形态、物理和力学性能进行了表征。进行了SEM分析、活/死染色、免疫染色试验以评估神经细胞的生长情况。将PLGA与MWCNTs混合导致支架的直径和孔隙率增加，并表现出更好的力学性能。结果表明，MWCNTs浓度较高的支架在培养8天后为神经细胞提供了更好的存活率，尤其是对星形胶质细胞的生长。这可能有助于治疗像多发性硬化症这样导致中枢神经系统脱髓鞘和轴突损伤的疾病。©2016威利期刊公司。《生物医学材料研究杂志》B部分：应用生物材料，105B：934 - 943，2017。
