Zhu Wei, Masood Fahed, O'Brien Joseph, Zhang Lijie Grace
Department of Mechanical and Aerospace Engineering, The George Washington University, Washington, DC, USA.
University of Maryland, Collage Park, MD, USA.
Nanomedicine. 2015 Apr;11(3):693-704. doi: 10.1016/j.nano.2014.12.001. Epub 2015 Jan 14.
Neural tissue engineering offers a promising avenue for repairing neural injuries. Advancement in nanotechnology and neural scaffold manufacturing strategies has shed light on this field into a new era. In this study, a novel tissue engineered scaffold, which possesses highly aligned poly-ε-caprolactone microfibrous framework and adjustable bioactive factor embedded poly (d, l-lactide-co-glycolide) core-shell nanospheres, was fabricated by combining electrospinning and electrospraying techniques. The fabricated nanocomposite scaffold has cell favorable nanostructured feature and improved hydrophilic surface property. More importantly, by incorporating core-shell nanospheres into microfibrous scaffold, a sustained bioactive factor release was achieved. Results show rat pheochromocytoma (PC-12) cell proliferation was significantly promoted on the nanocomposite scaffold. In addition, confocal microscope images illustrated that the highly aligned scaffold increased length of neurites and directed neurites extension along the fibers in both PC-12 and astrocyte cell lines, which indicates that the scaffold is promising for guiding neural tissue growth and regeneration. From the clinical editor: In an attempt to direct neural cell growth, biomimetic neural scaffold was produced by electrospinning integrated with co-axial electrospraying techniques. In-vitro data provided a framework for future designs for neuronal regeneration.
神经组织工程为修复神经损伤提供了一条充满希望的途径。纳米技术和神经支架制造策略的进步为该领域开启了一个新时代。在本研究中，通过结合静电纺丝和电喷雾技术，制备了一种新型组织工程支架，其具有高度排列的聚ε-己内酯微纤维框架和嵌入生物活性因子的可调节聚（d，l-丙交酯-共-乙交酯）核壳纳米球。所制备的纳米复合支架具有有利于细胞的纳米结构特征和改善的亲水性表面性质。更重要的是，通过将核壳纳米球纳入微纤维支架，实现了生物活性因子的持续释放。结果表明，纳米复合支架上大鼠嗜铬细胞瘤（PC-12）细胞的增殖显著促进。此外，共聚焦显微镜图像显示，高度排列的支架增加了神经突的长度，并引导PC-12和星形胶质细胞系中的神经突沿纤维延伸，这表明该支架在引导神经组织生长和再生方面具有潜力。临床编辑评论：为了引导神经细胞生长，通过将静电纺丝与同轴电喷雾技术相结合制备了仿生神经支架。体外数据为未来神经元再生设计提供了一个框架。
