Pan L X, Beverley P C, Bobrow L G, Swallow D M, Isaacson P G
Department of Histopathology, University College and Middlesex Medical School, London.
Histochem J. 1989 Nov;21(11):638-44. doi: 10.1007/BF01002483.
Purified lactate dehydrogenase (LDH) isoenzyme 1 (H or B subunits) and isoenzyme 5 (M or A subunits) were used to prepare monoclonal antibodies (MAb) suitable for immunohistochemical detection on formalin fixed paraffin-embedded tissue sections. In the initial fusions, screening of the antibodies was based on enzyme linked immunosorbent assay (ELISA) against the immunogens. None of the antibodies obtained was satisfactory. There were various problems related to specificity, crossreactivity, affinity and also the properties of the monoclonal antibody itself. Using a combined system involving more than one method for screening, two suitable monoclonal antibodies, MAb65 (to H-type LDH) and MAb25 (to M-type LDH) were selected. Both antibodies reacted specifically with corresponding LDH isoenzymes as shown in a series of tests. Their reactivity in sections of formalin fixed paraffin-embedded tissue indicated that both antibodies are suitable reagents for immunohistochemical studies.
纯化的乳酸脱氢酶（LDH）同工酶1（H或B亚基）和同工酶5（M或A亚基）被用于制备适合在福尔马林固定石蜡包埋组织切片上进行免疫组织化学检测的单克隆抗体（MAb）。在最初的融合实验中，抗体筛选基于针对免疫原的酶联免疫吸附测定（ELISA）。所获得的抗体均不理想。存在各种与特异性、交叉反应性、亲和力以及单克隆抗体本身特性相关的问题。通过使用涉及多种筛选方法的组合系统，选择了两种合适的单克隆抗体，即MAb65（针对H型LDH）和MAb25（针对M型LDH）。如一系列测试所示，两种抗体均与相应的LDH同工酶发生特异性反应。它们在福尔马林固定石蜡包埋组织切片中的反应性表明，这两种抗体都是免疫组织化学研究的合适试剂。
