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用于鉴定大肠杆菌O157的商用乳胶玻片试验评估

Evaluation of commercial latex slide test for identifying Escherichia coli O157.

作者信息

Chapman P A

机构信息

Public Health Laboratory, Northern General Hospital, Sheffield.

出版信息

J Clin Pathol. 1989 Oct;42(10):1109-10. doi: 10.1136/jcp.42.10.1109.

Abstract

A commercial latex slide test (Oxoid DR 620) for the identification of Escherichia coli O157 was compared with a standard tube agglutination method using E coli O157 antiserum (Difco). Thirty isolates previously confirmed as E coli O157 were positive by both methods: 30 isolates of E coli of serogroups other than O157, 30 isolates of non-sorbitol fermenting organisms from various genera, and one isolate of E hermanii were all negative by both methods. It is concluded that the latex slide test offers a rapid and economical alternative to tube agglutination for the identification of E coli O157.

摘要

将一种用于鉴定大肠杆菌O157的商用乳胶玻片试验(Oxoid DR 620)与使用大肠杆菌O157抗血清(Difco)的标准试管凝集法进行了比较。之前已确认为大肠杆菌O157的30个分离株通过两种方法均呈阳性:30个O157以外血清群的大肠杆菌分离株、30个来自不同属的非山梨醇发酵菌分离株以及1个赫尔曼埃希菌分离株通过两种方法均为阴性。得出结论,乳胶玻片试验为鉴定大肠杆菌O157提供了一种快速且经济的替代试管凝集的方法。

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False positive identifications of Escherichia coli O157.
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