March S B, Ratnam S
Newfoundland and Labrador Public Health Laboratory, St. John's, Canada.
J Clin Microbiol. 1989 Jul;27(7):1675-7. doi: 10.1128/jcm.27.7.1675-1677.1989.
The value of a latex agglutination test (Escherichia coli O157 latex test; Oxoid Ltd.) for rapid presumptive detection of E. coli serotype O157:H7 was determined by laboratory trials and during an outbreak of hemorrhagic colitis. The latex test was found to be a simple, highly efficient and reliable test in detecting E. coli O157:H7 with 100% sensitivity and specificity. It was also found that sorbitol-MacConkey agar cultures were not as useful for food samples as they were for fecal specimens in screening for E. coli O157:H7, but the use of the latex screen was particularly efficient in this setting.
通过实验室试验以及在一次出血性结肠炎暴发期间,对用于快速初步检测大肠杆菌O157:H7血清型的乳胶凝集试验(大肠杆菌O157乳胶试验;Oxoid有限公司)的价值进行了测定。发现乳胶试验在检测大肠杆菌O157:H7方面是一种简单、高效且可靠的试验,敏感性和特异性均为100%。还发现,在筛查大肠杆菌O157:H7时,山梨醇麦康凯琼脂培养物对食品样本的作用不如对粪便标本的作用大,但在此情况下使用乳胶筛查特别有效。