Han Kaikai, Zhao Dongmin, Liu Yuzhuo, Huang Xinmei, Yang Jing, Liu Qingtao, An Fengjiao, Li Yin
Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, National Center for Engineering Research of Veterinary Bio-products, Nanjing, Jiangsu Province, PR China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, PR China.
Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, National Center for Engineering Research of Veterinary Bio-products, Nanjing, Jiangsu Province, PR China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, PR China.
Res Vet Sci. 2016 Feb;104:174-80. doi: 10.1016/j.rvsc.2015.09.011. Epub 2015 Sep 18.
Tembusu virus (TMUV) is a newly emerging pathogenic flavivirus that is causing massive economic loss in Chinese poultry industry; until now, there is no effective vaccine or drug for its prevention. Epitope-based vaccination is a promising approach to achieve protective immunity and to avoid immunopathology. In present study, based on in silico epitope selection, we optimized and proposed a polytope DNA vaccine (pVAX1-rTEM) consisting B-cell and T cell epitopes from the TMUV envelope (E) protein. The immunogenicity and protective efficacy of constructed polytope DNA vaccine was assessed by in vitro and in vivo experiments. In in vitro assays, the expressed pVAX1-rTEM showed reactivity with Tembusu positive serum. Its protective efficacy against TMUV infection was evaluated in ducks. The results showed that pVAX1-rTEM was highly immunogenic and could elicit high titer neutralizing antibodies and cell-mediated immune responses. These results indicate that pVAX1-rTEM may be a promising candidate vaccine for prevention of TMUV infection.
坦布苏病毒（TMUV）是一种新出现的致病性黄病毒，正在给中国家禽业造成巨大经济损失；到目前为止，尚无有效的预防疫苗或药物。基于表位的疫苗接种是实现保护性免疫并避免免疫病理的一种有前景的方法。在本研究中，基于计算机模拟表位选择，我们优化并提出了一种多表位DNA疫苗（pVAX1-rTEM），其包含来自TMUV包膜（E）蛋白的B细胞和T细胞表位。通过体外和体内实验评估构建的多表位DNA疫苗的免疫原性和保护效力。在体外试验中，表达的pVAX1-rTEM与坦布苏阳性血清发生反应。在鸭中评估了其对TMUV感染的保护效力。结果表明，pVAX1-rTEM具有高度免疫原性，可引发高滴度中和抗体和细胞介导的免疫反应。这些结果表明，pVAX1-rTEM可能是预防TMUV感染的一种有前景的候选疫苗。
