College of Animal Science and Technology, Anhui Agricultural University, Hefei, 230036, China.
Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 518 Ziyue Rd, Shanghai, 200241, China.
Virol J. 2018 Sep 14;15(1):140. doi: 10.1186/s12985-018-1053-0.
Duck Tembusu virus (DTMUV), a pathogenic flavivirus, emerged in China since 2010 and causing huge economic loss in the Chinese poultry industry. Although several vaccines have been reported to control DTMUV disease, few effective vaccines are available and new outbreaks were continuously reported. Thus, it is urgently to develop a new effective vaccine for prevention of this disease.
In this study, a suicidal DNA vaccine based on a Semliki Forest virus (SFV) replicon and DTMUV E glycoprotein gene was constructed and the efficacy of this new vaccine was assessed according to humoral and cell-mediated immune responses as well as protection against the DTMUV challenge in ducklings.
Our results showed that the recombinant SFV replicon highly expressed E glycoprotein in DEF cells. After intramuscular injection of this new DNA vaccine in ducklings, robust humoral and cellular immune responses were observed in all immunized ducklings. Moreover, all ducklings were protected against challenge with the virulent DTMUV AH-F10 strain.
In conclusion, we demonstrate that this suicidal DNA vaccine is a promising candidate facilitating the prevention of DTMUV infection.
鸭坦布苏病毒（DTMUV）是一种致病性黄病毒，自 2010 年在中国出现以来，给中国家禽业造成了巨大的经济损失。尽管已经报道了几种疫苗来控制 DTMUV 病，但可用的有效疫苗很少，新的疫情仍在不断报告。因此，迫切需要开发一种新的有效疫苗来预防这种疾病。
在这项研究中，构建了一种基于辛德毕斯森林病毒（SFV）复制子和 DTMUV E 糖蛋白基因的自杀 DNA 疫苗，并根据鸭的体液和细胞免疫反应以及对 DTMUV 攻毒的保护作用来评估这种新疫苗的功效。
我们的结果表明，重组 SFV 复制子在 DEF 细胞中高度表达 E 糖蛋白。在给鸭肌肉注射这种新型 DNA 疫苗后，所有免疫鸭均产生强烈的体液和细胞免疫反应。此外，所有鸭均能抵抗强毒 DTMUV AH-F10 株的攻击。
总之，我们证明这种自杀 DNA 疫苗是一种有前途的候选疫苗，有助于预防 DTMUV 感染。
