College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.
Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China.
Genes (Basel). 2021 Apr 17;12(4):589. doi: 10.3390/genes12040589.
The development of skeletal muscle is a highly ordered and complex biological process. Increasing evidence has shown that noncoding RNAs, especially long-noncoding RNAs (lncRNAs) and microRNAs, play a vital role in the development of myogenic processes. In this study, we observed that lncMyoD regulates myogenesis and changes myofiber-type composition. miR-370-3p, which is directly targeted by lncMyoD, promoted myoblast proliferation and inhibited myoblast differentiation in the C2C12 cell line, which serves as a valuable model for studying muscle development. In addition, the inhibition of miR-370-3p promoted fast-twitch fiber transition. Further analysis indicated that acyl-Coenzyme A dehydrogenase, short/branched chain (ACADSB) is a target gene of miR-370-3p, which is also involved in myoblast differentiation and fiber-type transition. Furthermore, our data suggested that miR-370-3p was sponged by lncMyoD. In contrast with miR-370-3p, lncMyoD promoted fast-twitch fiber transition. Taken together, our results suggest that miR-370-3p regulates myoblast differentiation and muscle fiber transition and is sponged by lncMyoD.
骨骼肌的发育是一个高度有序和复杂的生物学过程。越来越多的证据表明，非编码 RNA，特别是长非编码 RNA（lncRNA）和 microRNA，在肌生成过程的发育中起着至关重要的作用。在这项研究中，我们观察到 lncMyoD 调节肌生成并改变肌纤维型组成。lncMyoD 直接靶向的 miR-370-3p 促进 C2C12 细胞系中的成肌细胞增殖并抑制成肌细胞分化，C2C12 细胞系是研究肌肉发育的有价值模型。此外，miR-370-3p 的抑制促进了快肌纤维的转变。进一步的分析表明，酰基辅酶 A 脱氢酶，短/支链（ACADSB）是 miR-370-3p 的靶基因，它也参与成肌细胞分化和纤维型转变。此外，我们的数据表明 miR-370-3p 被 lncMyoD 海绵吸附。与 miR-370-3p 相反，lncMyoD 促进快肌纤维的转变。综上所述，我们的研究结果表明，miR-370-3p 调节成肌细胞分化和肌肉纤维的转变，并被 lncMyoD 海绵吸附。
