Vitreoretinal and Pathology Unit, Department of Ophthalmology, University Eye Hospital, Munich, Germany.
Retina. 2011 May;31(5):977-81. doi: 10.1097/IAE.0b013e3181f44282.
To demonstrate the incidence of pores in the inner limiting membrane (ILM) in flat-mounted ILM specimens and to show the immunocytochemical properties of cellular proliferation associated with them.
One hundred and twelve ILM specimens from patients with idiopathic macular holes were flat mounted and screened for pores. The ILM was assessed by phase-contrast and interference microscopy. Various antibodies were used against glial cells, hyalocytes, and retinal pigment epithelial cells.
In total, only three pores were found. They were characterized by a full-thickness defect of the ILM with irregular borders and cellular proliferation on the ILM. Glial cells were seen in direct association with the ILM pore. Glial cell and hyalocyte markers were the main immunologic features observed.
Inner limiting membrane pores are a rare finding. Glial cells are likely to cross the ILM through these pores on their way from the retina to the vitreoretinal border. Given the frequency of cellular proliferation and the rare finding of ILM pores, other pathways have to be considered in playing the leading role in epiretinal proliferation. They might include migration of cells through ILM thinning along retinal vessels and proliferation of vitreous cortex hyalocytes at the vitreoretinal border.
展示在平铺的内界膜（ILM）标本中孔的发生率，并显示与孔相关的细胞增殖的免疫细胞化学特性。
对 112 例特发性黄斑裂孔患者的 ILM 标本进行平铺并筛选孔。通过相差和干涉显微镜评估 ILM。使用针对神经胶质细胞、玻璃体细胞和视网膜色素上皮细胞的各种抗体。
总共仅发现 3 个孔。它们的特征是 ILM 全层缺陷，边界不规则，ILM 上有细胞增殖。在 ILM 孔中可以看到神经胶质细胞与 ILM 的直接关联。观察到的主要免疫特征是神经胶质细胞和玻璃体细胞标志物。
内界膜孔是一种罕见的发现。神经胶质细胞可能在从视网膜到玻璃体视网膜边界的过程中通过这些孔穿过 ILM。鉴于细胞增殖的频率和 ILM 孔的罕见发现，在视网膜前增殖中发挥主导作用的其他途径必须被考虑。它们可能包括细胞通过沿视网膜血管变薄的 ILM 的迁移以及在玻璃体视网膜边界处的玻璃体皮质玻璃体细胞的增殖。
