Xu Jiping, Zhong Haibin, Cui Ling, Lan Qianqian, Chen Lifei, He Wenjing, Wu Yu, Jiang Li, Huang Hui, Zhao Xin, Li Li, Zeng Siming, Li Min, Xu Fan
Department of Ophthalmology, People's Hospital of Guangxi Zhuang Autonomous Region, Nanning, Guangxi, People's Republic of China.
Oncotarget. 2017 May 30;8(22):35532-35541. doi: 10.18632/oncotarget.16683.
The aims of the present study were to investigate the expression and distribution of Wild-type p53-induced phosphatase 1 (Wip1) in diabetic patients with proliferative diabetic retinopathy (PDR) with epiretinal membranes (ERMs) meanwhile analyze the colocalization of Wip1 and nuclear factor kappa-B (NF-κB) p65 in ERMs.
ERMs samples were collected from patients with PDR (PDR group) or non-diabetic patients with idiopathic epiretinal membranes (iERMs) (control group) during pars plana vitrectomy. Real-Time PCR analysis was carried out to examine the mRNA expression of Wip1 in ERMs. Immunohistochemical analysis and Immunofluorescent analysis were performed to detect the protein expression of Wip1 in ERMs. Double immunofluorescent staining was performed to detect the colocalization of Wip1 and glial fibrillary acidic protein (GFAP) (retinal glial cells marker), also Wip1 and NF-κB.
ERMs were obtained from 17 eyes of 17 patients with PDR (the PDR group) and 9 eyes of 9 nondiabetic patients (the control group) with iERMs. Our results showed high expression levels of Wip1 mRNAs in ERMs after PDR, but low in iERMs. In addition, both immunohistochemistry and immunofluorescence assay showed strong immunoreactivity for Wip1 in PDR ERMs. Furthermore, Wip1 and GFAP were coexpressed in PDR membranes. Finally, the expression of Wip1 was paralleled with NF-κB.
These data support the notion that Wip1 contributes to the formation of the ERMs in PDR membranes via NF-κB signaling.
本研究旨在探讨野生型p53诱导磷酸酶1（Wip1）在伴有视网膜前膜（ERM）的增殖性糖尿病视网膜病变（PDR）糖尿病患者中的表达及分布情况，同时分析Wip1与核因子κB（NF-κB）p65在ERM中的共定位情况。
在玻璃体切割手术过程中，从PDR患者（PDR组）或非糖尿病特发性视网膜前膜患者（iERM组，对照组）采集ERM样本。采用实时定量聚合酶链反应（Real-Time PCR）分析检测ERM中Wip1的mRNA表达。进行免疫组织化学分析和免疫荧光分析以检测ERM中Wip1的蛋白表达。采用双重免疫荧光染色检测Wip1与胶质纤维酸性蛋白（GFAP，视网膜神经胶质细胞标志物）的共定位情况，以及Wip1与NF-κB的共定位情况。
从17例PDR患者的17只眼中（PDR组）以及9例非糖尿病iERM患者的9只眼中（对照组）获取ERM。我们的结果显示，PDR后ERM中Wip1 mRNA表达水平较高，而iERM中较低。此外，免疫组织化学和免疫荧光检测均显示PDR的ERM中Wip1具有强免疫反应性。此外，Wip1与GFAP在PDR膜中共表达。最后，Wip1的表达与NF-κB平行。
这些数据支持以下观点，即Wip1通过NF-κB信号通路促进PDR膜中ERM的形成。
