量化多聚体膜受体中的协同亚基作用。
Quantifying the cooperative subunit action in a multimeric membrane receptor.
作者信息
Wongsamitkul Nisa, Nache Vasilica, Eick Thomas, Hummert Sabine, Schulz Eckhard, Schmauder Ralf, Schirmeyer Jana, Zimmer Thomas, Benndorf Klaus
机构信息
Institut für Physiologie II, Universitätsklinikum Jena, Friedrich-Schiller-Universität Jena, 07743 Jena, Germany.
Hochschule Schmalkalden, Fakultät Elektrotechnik, Blechhammer, 98574 Schmalkalden, Germany.
出版信息
Sci Rep. 2016 Feb 9;6:20974. doi: 10.1038/srep20974.
In multimeric membrane receptors the cooperative action of the subunits prevents exact knowledge about the operation and the interaction of the individual subunits. We propose a method that permits quantification of ligand binding to and activation effects of the individual binding sites in a multimeric membrane receptor. The power of this method is demonstrated by gaining detailed insight into the subunit action in olfactory cyclic nucleotide-gated CNGA2 ion channels.
在多聚体膜受体中，亚基的协同作用使得我们无法确切了解各个亚基的运作及相互作用情况。我们提出了一种方法，该方法能够对多聚体膜受体中各个结合位点的配体结合及激活效应进行定量分析。通过深入了解嗅觉环核苷酸门控CNGA2离子通道中的亚基作用，证明了该方法的有效性。
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