Chen Jianwei, Wu Qihao, Hawas Usama W, Wang Hong
College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou, 310014, China.
Marine Chemistry Department, Faculty of Marine Science, King Abdulaziz University, P.O. Box 80207, Jeddah, 21589, Kingdom of Saudi Arabia.
Appl Microbiol Biotechnol. 2016 Apr;100(7):2953-65. doi: 10.1007/s00253-016-7357-3. Epub 2016 Feb 10.
Natural products are an important source of modern medical development, e.g., antibiotics, anticancers, immune modulators, etc. and will continue to be a powerful driving force for the discovery of novel potential drugs. In the heterologous hosts, natural products are biosynthesized using dedicated metabolic networks. By gene engineering, pathway reconstructing, and enzyme engineering, metabolic networks can be modified to synthesize novel compounds containing enhanced structural feature or produce a large quantity of known valuable bioactive compounds. The review introduces some important technical platforms and relevant examples of genetic regulation and manipulation to improve natural product titers or drive novel secondary metabolite discoveries.
天然产物是现代医学发展的重要来源，例如抗生素、抗癌药物、免疫调节剂等，并且将继续成为发现新型潜在药物的强大驱动力。在异源宿主中，天然产物通过专门的代谢网络进行生物合成。通过基因工程、途径重构和酶工程，可以对代谢网络进行修饰，以合成具有增强结构特征的新型化合物或大量生产已知的有价值的生物活性化合物。本文综述介绍了一些重要的技术平台以及基因调控和操作的相关实例，以提高天然产物产量或推动新型次生代谢产物的发现。
