Mathankumar Marimuthu, Tamizhselvi Ramasamy, Manickam Venkatraman, Purohit Gaurav
School of Biosciences and Technology, VIT University, Vellore, Tamil Nadu, India.
Toxicol Int. 2015 Jan-Apr;22(1):114-8. doi: 10.4103/0971-6580.172269.
The main objective of this preliminary study is to confirm the synergistic anticarcinogenic potential of Vitex trifolia and Triticum aestivum ethanolic extracts.
Rat hepatic microsomal degranulation is a short - term technique that has been used for the detection of potential chemical carcinogens, in vitro. The present study has been carried out to study the inhibition of ribosome- membrane disruption against 3, 8-Diamino-5-ethyl-6-pheylphenanthridinium bromide (EB), as the degranulating agent, by measuring the RNA/protein ratios of microsomal membranes in the presence or absence of V.trifolia and T. aestivum extracts. These two extracts were further evaluated for cytotoxic effect in HCT 116 and A549 cell lines.
V. trifolia and T. aestivum protects hepatic microsomes against the degranulatory attack by the carcinogen EB showed a significant reduction in the proliferation of the HCT 116 and A549 cancer cell lines.
The ethanolic extracts of the plants, V. trifolia and T. aestivum individually possessed anti-degranulatory potential. Importantly they act synergistically, possess appreciable anticarcinogenic properties, based on their ability to inhibit EB induced liver microsomal degranulation. Further these extracts inhibit cell proliferation of cancer cell lines.
本初步研究的主要目的是证实三叶牡荆和普通小麦乙醇提取物的协同抗癌潜力。
大鼠肝微粒体脱粒是一种已用于体外检测潜在化学致癌物的短期技术。本研究旨在通过测量在有或没有三叶牡荆和普通小麦提取物的情况下微粒体膜的RNA/蛋白质比率，研究对作为脱粒剂的3,8-二氨基-5-乙基-6-苯基菲啶溴化物（EB）的核糖体-膜破坏的抑制作用。进一步评估这两种提取物对HCT 116和A549细胞系的细胞毒性作用。
三叶牡荆和普通小麦保护肝微粒体免受致癌物EB的脱粒攻击，HCT 116和A549癌细胞系的增殖显著降低。
三叶牡荆和普通小麦这两种植物的乙醇提取物各自具有抗脱粒潜力。重要的是，基于它们抑制EB诱导的肝微粒体脱粒的能力，它们协同作用，具有明显的抗癌特性。此外，这些提取物抑制癌细胞系的细胞增殖。
