Manjunatha B K, Vidya S M
Department of Biotechnology, the Oxford College of Engineering, Bommanahalli, Bangalore-560 068, India.
Indian J Pharm Sci. 2008 Mar-Apr;70(2):241-5. doi: 10.4103/0250-474X.41466.
Aqueous and ethanol extracts of leaf of Vitex trifolia was investigated for hepatoprotective activity against carbon tetrachloride induced liver damage. To assess the hepatoprotective activity of the extracts, various biochemical parameters viz., total bilirubin, total protein, alanine transaminase, aspartate transaminase and alkaline phosphatase activities were determined. Results of the serum biochemical estimations revealed significant reduction in total bilirubin and serum marker enzymes and increase in total protein in the animals treated with ethanol and aqueous extracts. However significant rise in these serum enzymes and decrease in total protein level was noticed in CCl4 treated group indicating the hepatic damage. The hepatoprotective activity is also supported by histological studies of liver tissue. Histology of the liver tissue treated with ethanol and aqueous extracts showed normal hepatic architecture with few fatty lobules. Hence the present study revealed that Vitex trifolia could afford significant protection against CCl(4) induced hepatocellular injury.
对蔓荆子叶的水提取物和乙醇提取物进行了研究，以考察其对四氯化碳诱导的肝损伤的保肝活性。为评估提取物的保肝活性，测定了各种生化参数，即总胆红素、总蛋白、丙氨酸转氨酶、天冬氨酸转氨酶和碱性磷酸酶活性。血清生化测定结果显示，用乙醇提取物和水提取物处理的动物，其总胆红素和血清标志物酶显著降低，总蛋白增加。然而，在四氯化碳处理组中，这些血清酶显著升高，总蛋白水平降低，表明存在肝损伤。肝组织的组织学研究也支持了保肝活性。用乙醇提取物和水提取物处理的肝组织组织学显示肝结构正常，仅有少量脂肪小叶。因此，本研究表明蔓荆子叶对四氯化碳诱导的肝细胞损伤具有显著的保护作用。
