Lam E, Packer L
Arch Biochem Biophys. 1983 Mar;221(2):557-64. doi: 10.1016/0003-9861(83)90175-3.
Treatment of purple membranes isolated from Halobacterium halobium with nonionic detergents caused the following effects in addition to solubilizing bacteriorhodopsin: a blue shift of the absorption spectrum of bacteriorhodopsin; an increase in tryptophan fluorescence of about twofold; an enhanced rate of bleaching in the presence of hydroxylamine; and the disappearance of the negative exciton band in the CD spectrum at 600 nm. Crosslinking of purple membranes by glutaraldehyde can prevent solubilization of bacteriorhodopsin by detergents. However, only the effect on the CD spectrum by detergents can be inhibited by crosslinking of the purple membranes prior to detergent treatment. Photocycle kinetics studies revealed that the apparent pK of the slow component of the M412 decay was decreased by the detergent treatment while the rise time for M412 formation is accelerated two- to threefold. Crosslinking also did not prevent these effects. These results demonstrate that nonionic detergents, apart from their action to monomerize bacteriorhodopsin, also affect retinal-apoprotein interactions.
用非离子型去污剂处理从嗜盐菌中分离出的紫膜，除了使细菌视紫红质溶解外，还产生了以下效应：细菌视紫红质吸收光谱发生蓝移；色氨酸荧光增强约两倍；在羟胺存在下漂白速率加快；以及在600nm处圆二色光谱中负激子带消失。戊二醛对紫膜进行交联可防止去污剂溶解细菌视紫红质。然而，只有在去污剂处理之前对紫膜进行交联才能抑制去污剂对圆二色光谱的影响。光循环动力学研究表明，去污剂处理使M412衰减慢成分的表观pK值降低，而M412形成的上升时间加快了两到三倍。交联也不能阻止这些效应。这些结果表明，非离子型去污剂除了使细菌视紫红质单体化的作用外，还影响视黄醛-脱辅基蛋白的相互作用。
