Wang Bao-gui, Xu Hai-bo, Xu Feng, Zeng Zhe-ling, Wei Hua
a State Key Laboratory of Food Science and Engineering, Nanchang University, Nanchang, Jiangxi 330047, People's Republic of China.
b Tianjin Key Laboratory of Exercise Physiology and Sports Medicine, Department of Health and Exercise Science, Tianjin University of Sport, Tianjin 300381, People's Republic of China.
Can J Microbiol. 2016 Mar;62(3):249-62. doi: 10.1139/cjm-2015-0685. Epub 2015 Dec 15.
This study aimed to examine whether Bifidobacterium bifidum ATCC 29521, a species of colonic microflora in humans, is involved in the intestinal tract of mice. This study was also conducted to determine the antioxidant activity of this species by evaluating different microbial populations and reactive oxygen species isolated from feces and intestinal contents for 28 days of oral administration. Microbial diversities were assessed through bacterial culture techniques, PCR-DGGE, and real-time PCR. This study showed that the intake of B. bifidum ATCC 29521 significantly (p < 0.05) improved the ecosystem of the intestinal tract of BALB/c mice by increasing the amount of probiotics (Lactobacillus intestinalis and Lactobacillus crispatus) and by reducing unwanted bacterial populations (Enterobacter, Escherichia coli). Antioxidative activities of incubated cell-free extracts were evaluated through various assays, including the scavenging ability of DPPH radical (64.5% and 67.54% (p < 0.05), respectively, at 21 days in nutrients and 28 days in MRS broth), superoxide anion, and hydroxyl radical (85% and 61.5% (p < 0.05), respectively, at intestinal contents in nutrients and 21 days in MRS broth). Total reducing power (231.5 μmol/L (p < 0.05), 14 days in MRS broth) and mRNA level of genes related to oxidative stress were also determined. Results indicated that B. bifidum ATCC 29521 elicits a beneficial effect on murine gut microbiota and antioxidant activities compared with the control samples. This species can be considered as a potential bioresource antioxidant to promote health. Bifidobacterium bifidum ATCC 29521 may also be used as a promising material in microbiological and food applications.
本研究旨在检测人类结肠微生物群中的两歧双歧杆菌ATCC 29521是否参与小鼠肠道活动。本研究还通过评估口服给药28天后从粪便和肠道内容物中分离出的不同微生物种群和活性氧，来测定该菌株的抗氧化活性。通过细菌培养技术、PCR-DGGE和实时PCR评估微生物多样性。本研究表明，摄入两歧双歧杆菌ATCC 29521可显著（p < 0.05）改善BALB/c小鼠的肠道生态系统，增加益生菌（肠乳杆菌和卷曲乳杆菌）数量，并减少有害细菌种群（肠杆菌、大肠杆菌）。通过各种检测方法评估无细胞提取物的抗氧化活性，包括DPPH自由基清除能力（在营养培养基中培养21天和在MRS肉汤中培养28天时，清除率分别为64.5%和67.54%（p < 0.05））、超氧阴离子和羟基自由基清除能力（在营养培养基中的肠道内容物和在MRS肉汤中培养21天时，清除率分别为85%和61.5%（p < 0.05））。还测定了总还原能力（在MRS肉汤中培养14天时为231.5 μmol/L（p < 0.05））以及与氧化应激相关基因的mRNA水平。结果表明，与对照样品相比，两歧双歧杆菌ATCC 29521对小鼠肠道微生物群和抗氧化活性具有有益影响。该菌株可被视为促进健康的潜在生物资源抗氧化剂。两歧双歧杆菌ATCC 29521也可能作为微生物学和食品应用中的一种有前景的材料。
