Faculty of Yasar Dogu Sports Sciences, Ondokuz Mayıs University, Samsun 55139, Turkey.
Pediatrics-Gastroenterology, University of Chicago Medicine, Chicago, IL 60637, USA.
Nutrients. 2022 Aug 29;14(17):3555. doi: 10.3390/nu14173555.
Intestinal microbiota play a role in the health and performance of athletes, and can be influenced by probiotics. Thus, in this study, we aimed to investigate the effect of the use of probiotics combined with chronic exercise on the thiol/disulfide homeostasis, a novel marker of oxidative stress.
Male Wistar rats were randomly divided into four groups: control (Cn), exercise (Ex), probiotics (P), and probiotics + exercise (PEx). A capsule containing 6 × 10 CFU of , and was given daily for eight weeks to all the experimental animals. The total thiol (TT, μmol/L) and native thiol (NT, μmol/L) concentrations were measured to determine the oxidative stress parameters. The dynamic disulfide (DD, %), reduced thiol (RT, %), oxidized thiol (OT, %), and thiol oxidation reduction (TOR, %) ratios were analyzed.
The TT level was found to be significantly higher in the Ex group (p = 0.047, η = 0.259). The DD level, a marker of oxidation, was significantly lower in the PEx group (p = 0.042, η = 0.266); the highest value of this parameter was found in the Ex group. The use of probiotics alone had no effect on thiol/disulfide homeostasis.
We showed, for the first time, that probiotics administered "with exercise" decreased dynamic disulfide and significantly reduced oxidative damage. Therefore, we speculate that the use of probiotics in sports involving intense exercise might be beneficial to reduce oxidative stress.
肠道微生物群在运动员的健康和表现中发挥作用，并且可以受到益生菌的影响。因此，在这项研究中，我们旨在研究使用益生菌联合慢性运动对硫醇/二硫键平衡（氧化应激的新标志物）的影响。
雄性 Wistar 大鼠随机分为四组：对照组（Cn）、运动组（Ex）、益生菌组（P）和益生菌+运动组（PEx）。所有实验动物每天都给予含有 6×10 CFU 的 、 和 的胶囊，持续八周。测量总硫醇（TT，μmol/L）和天然硫醇（NT，μmol/L）浓度以确定氧化应激参数。分析动态二硫键（DD，%）、还原硫醇（RT，%）、氧化硫醇（OT，%）和硫醇氧化还原（TOR，%）比值。
发现 Ex 组 TT 水平显著升高（p=0.047，η=0.259）。PEx 组氧化标志物 DD 水平显著降低（p=0.042，η=0.266）；该参数的最高值出现在 Ex 组。单独使用益生菌对硫醇/二硫键平衡没有影响。
我们首次表明，“与运动一起”给予益生菌可降低动态二硫键并显著降低氧化损伤。因此，我们推测在涉及剧烈运动的运动中使用益生菌可能有助于减少氧化应激。
