Abbasi Behzad, Abbasi Homayoun, Niroumand Hassan
Trauma Research Center, AJA University of Medical Sciences, Tehran, Iran.
Isfahan Fertility and Infertility Center, Isfahan, Iran.
Int J Reprod Biomed. 2021 Mar 21;19(3):235-244. doi: 10.18502/ijrm.v19i3.8571. eCollection 2021 Mar.
Idiopathic male infertility is often treated empirically. A recent body of evidence has indicated the association between pro prebiotics administration and improvement in semen parameters.
To assess the effect of FamiLact (probiotic + prebiotic) administration on male subjects with idiopathic infertility.
Fifty-six men with idiopathic male infertility were randomly/equally divided into two groups. Men in the case and control groups received 500 mg of FamiLact and an identical placebo for 80 days, respectively. A semen sample was obtained from each of the participants before initiation and after the termination of the treatment course. Samples underwent regular semen analysis and were further analyzed to assess the level of DNA damage (sperm chromatin structure assay), oxidative stress (BODIPY C11 staining), and protamine deficiency (chromomycin-A3 staining) in spermatozoa.
No significant difference was observed between the baseline values of both groups. After intervention, mean sperm concentration, motility, and normal morphology were significantly higher in the FamiLact group compared to the placebo group (p 0.05). In the FamiLact receivers, we detected improvement regarding the following parameters: concentration, motility, abnormal morphology, sperm lipid peroxidation, and DNA fragmentation (p 0.02). Likewise, in the placebo group, we noticed a decrease in the post-medication mean value of DNA fragmentation (p = 0.03) while observing no significant difference regarding other parameters.
FamiLact administration improves sperm concentration, motility, and abnormal morphology and decrease sperm DNA damage, possibly through alleviating oxidative stress in the seminal fluid.
特发性男性不育症通常采用经验性治疗。最近有大量证据表明，服用益生菌和益生元与精液参数改善之间存在关联。
评估服用FamiLact（益生菌+益生元）对特发性不育男性受试者的影响。
56名特发性男性不育患者被随机/平均分为两组。病例组和对照组男性分别服用500毫克FamiLact和相同的安慰剂，持续80天。在治疗疗程开始前和结束后，从每位参与者采集精液样本。样本进行常规精液分析，并进一步分析以评估精子中的DNA损伤水平（精子染色质结构分析）、氧化应激（BODIPY C11染色）和鱼精蛋白缺乏（放线菌素A3染色）情况。
两组的基线值之间未观察到显著差异。干预后，与安慰剂组相比，FamiLact组的平均精子浓度、活力和正常形态显著更高（p<0.05）。在服用FamiLact的受试者中，我们检测到以下参数有所改善：浓度、活力、异常形态、精子脂质过氧化和DNA片段化（p<0.02）。同样，在安慰剂组中，我们注意到用药后DNA片段化的平均值有所下降（p = 0.03），而其他参数未观察到显著差异。
服用FamiLact可改善精子浓度、活力和异常形态，并减少精子DNA损伤，可能是通过减轻精液中的氧化应激来实现的。
