Cohen A M
Proc Natl Acad Sci U S A. 1977 Jul;74(7):2899-903. doi: 10.1073/pnas.74.7.2899.
Neural crest cells obtained from Japanese quail and grown in vitro without other embryonic tissues differentiate into adrenergic cells. These cells show intense catecholamine-specific histochemical fluorescence, and some have long, varicose neuronal processes. Ultrastructural examination shows two populations of cells, one with small (about 90 nm) dense-core vesicles resembling principal sympathetic neurons and the other with larger (about 150 nm) dense-core granules resembling chromaffin or small intensely fluorescent cells. Neuronal cells without adrenergic characteristics are also present. These results are compatible with the hypothesis that a population of cells determined along neuronal lines exists in the neural crest prior to migration.
从日本鹌鹑获取的神经嵴细胞在无其他胚胎组织的情况下于体外培养，可分化为肾上腺素能细胞。这些细胞呈现强烈的儿茶酚胺特异性组织化学荧光，部分细胞具有长的、曲张的神经元突起。超微结构检查显示有两类细胞，一类具有小的（约90纳米）致密核心囊泡，类似于主要的交感神经元；另一类具有较大的（约150纳米）致密核心颗粒，类似于嗜铬细胞或小而强荧光细胞。也存在无肾上腺素能特征的神经元细胞。这些结果与以下假说相符，即在迁移之前神经嵴中存在一群沿神经元谱系确定的细胞。
