Cochard P, Goldstein M, Black I B
Proc Natl Acad Sci U S A. 1978 Jun;75(6):2986-90. doi: 10.1073/pnas.75.6.2986.
The ontogenetic pattern of noradrenergic differentiation in rat embryonic autonomic neuroblasts was defined in vivo. Noradrenergic specialization was examined by documenting the immunohistochemical appearance of tyrosine hydroxylase [Tyr-OH; tyrosine 3-monooxygenase; L-tyrosine,-tetrahydropteridine:oxygen oxidoreductase (3-hydroxylating), EC 1.14.16.2] and the development of histofluorescence due to catecholamine (CA). Tyr-OH and CA were undetectable in the dorsal neural crest or the ventrally migrating crest cells and first appeared at 12.5 days of gestation (36--37 somite stage) in sympathoblasts that had formed sympathetic ganglion primordia. Fluorescence intensity and the number of fluorescent cells increased progressively thereafter. In addition, Tyr-OH and CA transiently appeared in scattered presumptive neuroblasts in the gut. The enzyme and transmitter were first detectable at 11.5 days of gestation and thereafter decreased progressively so that, by 14.5 days, only rare cells were encountered. There was remarkable synchrony in the appearance and disappearance of Tyr-OH and CA. These observations suggest that a number of noradrenergic transmitter mechanisms develop simultaneously in the differentiating neuroblast. The relevance of these results to the elucidation of developmental regulatory mechanisms is discussed.
在体内确定了大鼠胚胎自主神经母细胞中去甲肾上腺素能分化的个体发生模式。通过记录酪氨酸羟化酶[酪氨酸3-单加氧酶；L-酪氨酸，四氢生物蝶呤：氧氧化还原酶（3-羟化），EC 1.14.16.2]的免疫组化表现以及儿茶酚胺（CA）引起的组织荧光的发展来研究去甲肾上腺素能特化。在背神经嵴或向腹侧迁移的嵴细胞中未检测到酪氨酸羟化酶和儿茶酚胺，它们首次出现在妊娠12.5天（36 - 37体节期）形成交感神经节原基的成神经细胞中。此后，荧光强度和荧光细胞数量逐渐增加。此外，酪氨酸羟化酶和儿茶酚胺短暂出现在肠道中散在的假定神经母细胞中。该酶和递质在妊娠11.5天首次可检测到，此后逐渐减少，以至于到14.5天时，仅能遇到罕见的细胞。酪氨酸羟化酶和儿茶酚胺的出现和消失存在显著的同步性。这些观察结果表明，在分化的神经母细胞中，多种去甲肾上腺素能递质机制同时发展。讨论了这些结果与阐明发育调控机制的相关性。
