Wang Chun-Chi, Wu Shou-Mei, Li Hung-Wen, Chang Huan-Tsung
Department of Chemistry, National Taiwan University, 1, Section 4, Roosevelt Road, Taipei, 10617, Taiwan.
School of Pharmacy, Kaohsiung Medical University, 100, Shih-Chuan 1st Road, Kaohsiung, 80708, Taiwan.
Chembiochem. 2016 Jun 16;17(12):1052-62. doi: 10.1002/cbic.201600014. Epub 2016 Apr 8.
Gold nanoparticles (AuNPs) are useful for diagnostic and biomedical applications, mainly because of their ease in preparation and conjugation, biocompatibility, and size-dependent optical properties. However, bare AuNPs do not possess specificity for targets. AuNPs conjugated with DNA aptamers offer specificity for various analytes, such as proteins and small molecules/ions. Although DNA aptamers themselves have therapeutic and target-recognizing properties, they are susceptible to degradation in vivo. When DNA aptamers are conjugated to AuNPs, their stability and cell uptake efficiency both increase, making aptamer-AuNPs suitable for biomedical applications. Additionally, drugs can be efficiently conjugated with DNA aptamer-AuNPs to further enhance their therapeutic efficiency. This review focuses on the applications of DNA aptamer-based AuNPs in several biomedical areas, including anticoagulation, anticancer, antibacterial, and antiviral applications.
金纳米颗粒（AuNPs）可用于诊断和生物医学应用，主要是因为它们易于制备和偶联、具有生物相容性以及尺寸依赖性光学特性。然而，裸金纳米颗粒对靶标不具有特异性。与DNA适配体偶联的金纳米颗粒对各种分析物具有特异性，如蛋白质和小分子/离子。尽管DNA适配体本身具有治疗和靶标识别特性，但它们在体内易降解。当DNA适配体与金纳米颗粒偶联时，其稳定性和细胞摄取效率都会提高，使得适配体-金纳米颗粒适用于生物医学应用。此外，药物可以有效地与DNA适配体-金纳米颗粒偶联，以进一步提高其治疗效率。本综述重点关注基于DNA适配体的金纳米颗粒在几个生物医学领域的应用，包括抗凝、抗癌、抗菌和抗病毒应用。
