Kanno Satomi, Cuyas Laura, Javot Hélène, Bligny Richard, Gout Elisabeth, Dartevelle Thibault, Hanchi Mohamed, Nakanishi Tomoko M, Thibaud Marie-Christine, Nussaume Laurent
Commissariat à l'Energie Atomique (CEA), Institut de Biologie Environnementale et de Biotechnologie, Laboratoire de Biologie du Developpement des Plantes; Centre National de la Recherche Scientifique (CNRS), Unité Mixte de Recherche 7265 Biologie Vegetale & Microbiologie Environnementale; Aix-Marseille Universite, Saint-Paul-lez-Durance, F-13108, France Graduate School of Agricultural and Life Sciences, the University of Tokyo, Yayoi, 1-1-1, Bunkyo-ku, Tokyo, 113-8657 Japan Biotechnology Research Center, the University of Tokyo, Yayoi, 1-1-1, Bunkyo-ku, Tokyo, 113-8657 Japan.
Commissariat à l'Energie Atomique (CEA), Institut de Biologie Environnementale et de Biotechnologie, Laboratoire de Biologie du Developpement des Plantes; Centre National de la Recherche Scientifique (CNRS), Unité Mixte de Recherche 7265 Biologie Vegetale & Microbiologie Environnementale; Aix-Marseille Universite, Saint-Paul-lez-Durance, F-13108, France.
Plant Cell Physiol. 2016 Apr;57(4):690-706. doi: 10.1093/pcp/pcv208. Epub 2016 Feb 9.
Phosphate (Pi) is a macronutrient that is essential for plant life. Several regulatory components involved in Pi homeostasis have been identified, revealing a very high complexity at the cellular and subcellular levels. Determining the Pi content in plants is crucial to understanding this regulation, and short real-time(33)Pi uptake imaging experiments have shown Pi movement to be highly dynamic. Furthermore, gene modulation by Pi is finely controlled by localization of this ion at the tissue as well as the cellular and subcellular levels. Deciphering these regulations requires access to and quantification of the Pi pool in the various plant compartments. This review presents the different techniques available to measure, visualize and trace Pi in plants, with a discussion of the future prospects.
磷酸盐（Pi）是植物生长必需的大量营养素。已鉴定出参与磷稳态的几种调节成分，这揭示了细胞和亚细胞水平上的高度复杂性。确定植物中的磷含量对于理解这种调节至关重要，短期实时（33）Pi吸收成像实验表明Pi的移动具有高度动态性。此外，Pi对基因的调节在组织以及细胞和亚细胞水平上通过该离子的定位得到精细控制。解读这些调节需要了解并量化植物不同区室中的磷库。本文综述了可用于测量、可视化和追踪植物中Pi的不同技术，并讨论了未来前景。
