用人牛磺胆酸钠共转运多肽重建的小鼠肝细胞系的乙型肝炎病毒感染
Hepatitis B Virus Infection of a Mouse Hepatic Cell Line Reconstituted with Human Sodium Taurocholate Cotransporting Polypeptide.
作者信息
Lempp Florian A, Qu Bingqian, Wang Yong-Xiang, Urban Stephan
机构信息
Department of Infectious Diseases, Molecular Virology, University Hospital Heidelberg, Heidelberg, Germany.
Key Laboratory of Medical Molecular Virology, Institute of Medical Microbiology, Shanghai Medical College, Fudan University, Shanghai, China.
出版信息
J Virol. 2016 Apr 14;90(9):4827-4831. doi: 10.1128/JVI.02832-15. Print 2016 May.
Abstract
Hepatitis B virus (HBV) enters hepatocytes via its receptor, human sodium taurocholate cotransporting polypeptide (hNTCP). So far, HBV infection has been achieved only in human hepatic cells reconstituted with hNTCP and not in cells of mouse origin. Here, the first mouse liver cell line (AML12) which gains susceptibility to HBV upon hNTCP expression is described. Thus, HBV infection of receptor-expressing mouse hepatocytes does not principally require a human cofactor but can be triggered by endogenous murine determinants.
摘要
乙型肝炎病毒（HBV）通过其受体人牛磺胆酸钠共转运多肽（hNTCP）进入肝细胞。到目前为止，仅在用hNTCP重构的人肝细胞中实现了HBV感染，而在小鼠来源的细胞中未实现。在此，描述了首个在表达hNTCP后对HBV易感的小鼠肝细胞系（AML12）。因此，表达受体的小鼠肝细胞的HBV感染原则上不需要人类辅助因子，而是可以由内源性小鼠决定因素触发。
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