UCA1作为一种竞争性内源性RNA发挥作用，以抑制上皮性卵巢癌转移。
UCA1 functions as a competing endogenous RNA to suppress epithelial ovarian cancer metastasis.
作者信息
Yang Yijun, Jiang Yi, Wan Yicong, Zhang Lin, Qiu Jiangnan, Zhou Shulin, Cheng Wenjun
机构信息
Department of Gynecology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, Jiangsu, China.
Department of Obstetrics and Gynecology, Zhongda Hospital Affiliated to Southeast University, Medical School, Southeast University, Nanjing, 210009, China.
出版信息
Tumour Biol. 2016 Aug;37(8):10633-41. doi: 10.1007/s13277-016-4917-1. Epub 2016 Feb 11.
Urothelial cancer associated 1 (UCA1) is an example of functional long noncoding RNAs involved in many biologic processes. However, little is known about the association between UCA1 expression and metastasis in epithelial ovarian cancer (EOC). Findings of this study confirmed that not only UCA1 was aberrantly upregulated in EOC tissues and cells, but also correlated with status of lymph node metastasis and FIGO stage. Furthermore, univariate and multivariate analyses showed that UCA1 was a prognostic factor for overall survival in EOC patients. In vitro, knockdown of UCA1 reduced the invasion and migration ability of EOC cells. The results showed that UCA1 could function as an endogenous sponge by directly binding to miR-485-5p. Depletion of UCA1 was involved in the downregulation of matrix metallopeptidase 14 (MMP14) expression, a target gene of miR-485-5p. In conclusion, our work indicates that UCA1 is a new prognostic biomarker for EOC, establishing a novel connection among UCA1, miR-485-5p, and MMP14 in EOC metastasis.
尿路上皮癌相关 1（UCA1）是参与许多生物学过程的功能性长链非编码 RNA 的一个例子。然而，关于 UCA1 表达与上皮性卵巢癌（EOC）转移之间的关联知之甚少。本研究结果证实，UCA1 不仅在 EOC 组织和细胞中异常上调，而且与淋巴结转移状态和国际妇产科联盟（FIGO）分期相关。此外，单因素和多因素分析表明，UCA1 是 EOC 患者总生存的一个预后因素。在体外，敲低 UCA1 可降低 EOC 细胞的侵袭和迁移能力。结果表明，UCA1 可通过直接结合 miR-485-5p 发挥内源性海绵的作用。UCA1 的缺失参与了基质金属肽酶 14（MMP14）表达的下调，MMP14 是 miR-485-5p 的靶基因。总之，我们的工作表明 UCA1 是 EOC 的一种新的预后生物标志物，在 EOC 转移中建立了 UCA1、miR-485-5p 和 MMP14 之间的新联系。
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