Choi Jin Woo, Kim Jung Hoon, Kim Hyo-Cheol, Choi Won Seok, Baek Song Yi, Lee Kyoungbun, Chung Jin Wook
Department of Radiology, Seoul National University Hospital, Seoul National University College of Medicine, 101 Daehak-ro, Jongno-gu, Seoul, 110-744, Republic of Korea.
Department of Pathology, Seoul National University Hospital, Seoul National University College of Medicine, Seoul, Republic of Korea.
Abdom Radiol (NY). 2016 Feb;41(2):257-64. doi: 10.1007/s00261-015-0591-9.
To compare tumor vascularity and hemodynamics in three rat hepatoma models: N1-S1 cells in Sprague-Dawley rats, McA-RH7777 cells in Sprague-Dawley rats, and 13762 MAT B III cells in F344 rats.
The three rat hepatoma models were induced in five rats per group. After confirming that the tumors grew up to 10 mm on magnetic resonance imaging, the rats underwent dynamic contrast-enhanced ultrasonography (DCE-US). Afterward, the rats were euthanized for histologic analyses. The Kruskal-Wallis test was used to compare the rat hepatoma models. Correlation coefficients were calculated between the microvessel density (MVD) and DCE-US parameters.
On DCE-US imaging, arterial enhancement and washout were demonstrated in all N1-S1 tumors, while persistent peripheral enhancement on arterial to portal phases was shown in all 13762 MAT B III tumors. The McA-RH7777 tumors presented diverse enhancement patterns on arterial and portal phases. There were no significant differences in DCE-US parameters among the three hepatoma groups, while MVD was correlated with peak intensity (r = 0.565, p = 0.044), mean transit time (r = -0.559, p = 0.047), and time to peak (r = - 0.617, p = 0.025) of individual rats. The necrosis ratio was significantly different between the models (p = 0.031); 13762 MAT B III showed a significantly higher necrosis ratio than N1-S1 (p < 0.050 by post hoc test).
The N1-S1 tumor may be suitable as a model to investigate hypervascular hepatic tumors of the liver in DCE-US such as hepatocellular carcinoma among the three tumors.
比较三种大鼠肝癌模型中的肿瘤血管生成和血流动力学，这三种模型分别为：在斯普拉格-道利大鼠中接种N1-S1细胞、在斯普拉格-道利大鼠中接种McA-RH7777细胞以及在F344大鼠中接种13762 MAT B III细胞。
每组五只大鼠构建三种大鼠肝癌模型。在磁共振成像确认肿瘤长至10毫米后，对大鼠进行动态对比增强超声检查（DCE-US）。之后，对大鼠实施安乐死以进行组织学分析。采用Kruskal-Wallis检验比较大鼠肝癌模型。计算微血管密度（MVD）与DCE-US参数之间的相关系数。
在DCE-US成像中，所有N1-S1肿瘤均表现出动脉期强化和廓清，而所有13762 MAT B III肿瘤在动脉期至门脉期均表现为周边持续强化。McA-RH7777肿瘤在动脉期和门脉期呈现出多样的强化模式。三种肝癌组之间的DCE-US参数无显著差异，而MVD与个体大鼠的峰值强度（r = 0.565，p = 0.044）、平均通过时间（r = -0.559，p = 0.047）以及达峰时间（r = - 0.617，p = 0.025）相关。模型之间的坏死率存在显著差异（p = 0.031）；13762 MAT B III的坏死率显著高于N1-S1（事后检验p < 0.050）。
在这三种肿瘤中，N1-S1肿瘤可能适合作为在DCE-US中研究肝脏富血供肝肿瘤（如肝细胞癌）的模型。
