Souza Caio Marcelo Cury, Pereira Junior Silvio Alves, Moraes Thaís da Silva, Damasceno Jaqueline Lopes, Amorim Mendes Suzana, Dias Herbert Júnior, Stefani Ricardo, Tavares Denise Crispim, Martins Carlos Henrique Gomes, Crotti Antônio Eduardo Miller, Mendes-Giannini Maria José Soares, Pires Regina Helena
Laboratório de Pesquisa em Microbiologia Aplicada, Universidade de Franca, Franca, SP, Brazil.
Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, São Paulo, Brazil.
Med Mycol. 2016 Jul 1;54(5):515-23. doi: 10.1093/mmy/myw003. Epub 2016 Feb 11.
Dental prosthesis supports Candida species growth and may predispose the oral cavity to lesions. C. tropicalis has emerged as a colonizer of prosthesis and has shown resistance to clinically used antifungal agents, which has increased the search for new antifungals. This work describes the effectiveness of fifteen essential oils (EOs) against C. tropicalis The EOs were obtained by hydrodistillation and were chemically characterized by gas chromatography-mass spectrometry. The antifungal activities of the EOs were evaluated by the microdilution method and showed that Pelargonium graveolens (Geraniaceae) (PG-EO) was the most effective oil. Geraniol and linalool were the major constituents of PG-EO. The 2,3-Bis-(2-Methoxy-4-Nitro-5-Sulfophenyl)-2H-Tetrazolium-5-Carboxanilide (XTT) assay showed that all the clinical C. tropicalis strains formed viable biofilms. Scanning electron microscopy examination of the biofilms revealed a complex architecture with basal layer of yeast cells and an upper layer of filamentous cells. Treatments with PG-EO, linalool, and geraniol significantly reduced the number of viable biofilm cells and inhibited biofilm formation after exposure for 48 h. PG-EO, geraniol, and linalool were not toxic to normal human lung fibroblasts (GM07492A) at the concentrations they were active against C. tropicalis Together, our results indicated that C. tropicalis is susceptible to treatment with PG-EO, geraniol, and linalool, which could become options to prevent or treat this infection.
假牙支持念珠菌属生长，并可能使口腔易患病变。热带念珠菌已成为假牙的定植菌，并已显示出对临床使用的抗真菌剂具有抗性，这增加了对新型抗真菌剂的探索。这项工作描述了15种精油（EOs）对热带念珠菌的有效性。这些精油通过水蒸馏获得，并通过气相色谱-质谱进行化学表征。通过微量稀释法评估了精油的抗真菌活性，结果表明香叶天竺葵（牻牛儿苗科）（PG-EO）是最有效的精油。香叶醇和芳樟醇是PG-EO的主要成分。2,3-双-（2-甲氧基-4-硝基-5-磺基苯基）-2H-四唑-5-羧酰胺（XTT）试验表明，所有临床热带念珠菌菌株均形成了有活力的生物膜。对生物膜的扫描电子显微镜检查显示出一种复杂的结构，其基础层为酵母细胞，上层为丝状细胞。用PG-EO、芳樟醇和香叶醇处理48小时后，可显著减少有活力的生物膜细胞数量并抑制生物膜形成。PG-EO、香叶醇和芳樟醇在对热带念珠菌有活性的浓度下对正常人肺成纤维细胞（GM07492A）无毒。总之，我们的结果表明，热带念珠菌对PG-EO、香叶醇和芳樟醇治疗敏感，这可能成为预防或治疗这种感染的选择。
