海南荚蒾中的细胞毒性降羽扇豆烷型三萜

Cytotoxic nor-dammarane triterpenes from Viburnum hainanense Merr. et Chun.

作者信息

Wang Wei, Song Juan, Shi GuoBing, Yu QingGong

机构信息

Department of gastroenterology, Affiliated Zhongshan Hospital of Dalian University, Dalian, Liaoning 110042, China.

Shenyang Pharmaceutical University, Shenyang 110016, China; Department of Pharmacy, General Hospital of Shenyang Military Area Command, Shenyang 110840, China.

出版信息

Fitoterapia. 2016 Apr;110:8-12. doi: 10.1016/j.fitote.2016.02.006. Epub 2016 Feb 8.

DOI:10.1016/j.fitote.2016.02.006

PMID:26869099

Abstract

Five new nor-dammarane triterpenes, 12β-O-acetyl-17β-hydoxy-3,15-dioxo-20,21,22,23,24,25,26,27-octanordammanran (1), 12β-hydoxy-17β-methoxy-3,15-dioxo-20,21,22,23,24,25,26,27-octanordammanran (2), 12β-O-acetyl-3,15-dioxo-17-en-20,21,22,23,24,25,26,27-octanordammanran (3), 12β-hydoxy-15α-O-acetyl-3-oxo-17-en-20,21,22,23,24,25,26,27-octanordammanran (4) and 3β-hydoxy-17-oxo-12-en-20,21,22,23,24,25,26,27-octanordammanran (5), were obtained from the ethanol extract of the whole plants of Viburnum hainanense Merr. et Chun. Structural elucidation of all the compounds was performed by spectral methods such as 1D and 2D NMR, IR, UV, and HRESIMS. The isolated compounds were tested in vitro for cytotoxic potential against seven tumor cell lines (Hep-2, SCL-1, CAL-27, UMSCC-1, Detroit-562, SCC-PKU, and TCA-83). As result, compounds 1 and 2 exhibited cytotoxic activities with IC50 values less than 10 μM.

摘要

从海南荚蒾全株乙醇提取物中获得了5个新的降达玛烷型三萜，分别为12β-O-乙酰基-17β-羟基-3,15-二氧代-20,21,22,23,24,25,26,27-八降达玛烷（1）、12β-羟基-17β-甲氧基-3,15-二氧代-20,21,22,23,24,25,26,27-八降达玛烷（2）、12β-O-乙酰基-3,15-二氧代-17-烯-20,21,22,23,24,25,26,27-八降达玛烷（3）、12β-羟基-15α-O-乙酰基-3-氧代-17-烯-20,21,22,23,24,25,26,27-八降达玛烷（4）和3β-羟基-17-氧代-12-烯-20,21,22,23,24,25,26,27-八降达玛烷（5）。所有化合物的结构均通过一维和二维核磁共振、红外光谱、紫外光谱和高分辨电喷雾电离质谱等光谱方法进行了鉴定。对分离得到的化合物进行了体外抗七种肿瘤细胞系（Hep-2、SCL-1、CAL-27、UMSCC-1、Detroit-562、SCC-PKU和TCA-83）的细胞毒性测试。结果表明，化合物1和2表现出细胞毒性活性，IC50值小于10 μM。