Bu Xuefeng, Wang Mubin, Zhang Jie, Liu Jun, Jia Lijuan, Liang Bing, Yan Yulan
Department of General Surgery, The Affiliated People's Hospital of Jiangsu University, Zhenjiang, Jiangsu 212001, P.R. China.
Department of Surgical Medicine, Clinical Medicine College of Jiangsu University, Zhenjiang, Jiangsu 212013, P.R. China.
Oncol Lett. 2016 Jan;11(1):287-292. doi: 10.3892/ol.2015.3890. Epub 2015 Nov 6.
The aim of the present study is to investigate the effect of a recombinant plasmid adenovirus (pAd) expressing human interferon-λ1 (hIFN-λ1) on the proliferation of the gastric adenocarcinoma cell line SGC-7901. For this purpose, human gastric adenocarcinoma SGC-7901 cells were infected with recombinant pAd-hIFN-λ1, pAd-LacZ and phosphate-buffered saline (PBS), respectively, and the subsequent effects on the proliferation of the infected cells were compared. Cell proliferation was evaluated by MTT assay, while mRNA and protein expression of hIFN-λ1 were detected by reverse transcription-polymerase chain reaction analysis and immunofluorescence assay, respectively. In addition, terminal deoxynucleotidyl transferase deoxyuridine triphosphate nick end labeling assay and flow cytometry were conducted to analyze the rate of cell apoptosis. The results indicated that the proliferation of gastric adenocarcinoma SGC-7901 cells was significantly inhibited by pAd-hIFN-λ1. Furthermore, the apoptosis rate and the mRNA and protein expression levels of hIFN-λ1 were higher in pAd-hIFN-λ1-transfected cells, compared with the pAd-LacZ and PBS control groups. In conclusion, recombinant pAd-hIFN-λ1 induced the expression of hIFN-λ1 in gastric adenocarcinoma SGC-7901 cells, and significantly inhibited cell proliferation by promoting apoptosis in these cancer cells.
本研究旨在探讨表达人干扰素λ1（hIFN-λ1）的重组质粒腺病毒（pAd）对胃腺癌细胞系SGC-7901增殖的影响。为此，分别用重组pAd-hIFN-λ1、pAd-LacZ和磷酸盐缓冲盐水（PBS）感染人胃腺癌SGC-7901细胞，并比较其对感染细胞增殖的后续影响。通过MTT法评估细胞增殖，同时分别通过逆转录-聚合酶链反应分析和免疫荧光法检测hIFN-λ1的mRNA和蛋白表达。此外，进行末端脱氧核苷酸转移酶脱氧尿苷三磷酸缺口末端标记法和流式细胞术分析细胞凋亡率。结果表明，pAd-hIFN-λ1显著抑制胃腺癌SGC-7901细胞的增殖。此外，与pAd-LacZ和PBS对照组相比，pAd-hIFN-λ1转染细胞的凋亡率以及hIFN-λ1的mRNA和蛋白表达水平更高。总之，重组pAd-hIFN-λ1诱导胃腺癌SGC-7901细胞中hIFN-λ1的表达，并通过促进这些癌细胞的凋亡显著抑制细胞增殖。
