Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Iran.
Neurogenic Inflammation Research Centre and Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
J Tradit Complement Med. 2015 Jan 29;6(1):89-96. doi: 10.1016/j.jtcme.2014.11.018. eCollection 2016 Jan.
Medicinal plants are noted for their many advantages including the ability to treat diseases such as cancer. In this study, we examined the antitumor effect of the medicinal plant Nigella sativa on the morphology, survival, and apoptosis of ACHN (human renal adenocarcinoma) and GP-293 (normal renal epithelial) cell lines. From a hydroalcoholic extract of N. sativa, n-hexane and ethyl acetate fractions were extracted. Cells were treated with various concentrations of total hydroalcholic extract and n-hexane and ethyl acetate fractions; cell viability, morphological changes, and apoptosis were then determined. Results were presented as mean ± standard error of the mean (SEM). One-way analysis of variance (ANOVA) was applied for the statistical analysis of the data. The total extract and the fractions in a dose- and time-dependent manner reduced the cell viability in ACHN with no effect on the GP-293 cell line. In addition, the total extract resulted in more morphological changes in the ACHN cells compared to the GP-293 cells. The effect of the total extract in inducing apoptosis after 48 hours in the ACHN cell line was greater than in GP-293. In addition, the effect of the two fractions was lower than the total extract at all used concentrations. Therefore, the effect of total extract and n-hexane and ethyl acetate fractions of N. sativa on cell viability and apoptosis in the ACHN cell line is greater than in the GP-293 cell line. However, the effect of the total extract is higher than either of the two fractions on their own.
药用植物以其诸多优点而闻名，包括能够治疗癌症等疾病。在这项研究中，我们研究了药用植物黑种草（Nigella sativa）对 ACHN（人肾腺癌）和 GP-293（正常肾上皮）细胞系的形态、存活和凋亡的抗肿瘤作用。从黑种草的水醇提取物中提取了正己烷和乙酸乙酯部分。用不同浓度的总水醇提取物和正己烷和乙酸乙酯部分处理细胞，然后测定细胞活力、形态变化和凋亡。结果以平均值±标准误差（SEM）表示。采用单因素方差分析（ANOVA）对数据进行统计学分析。总提取物和各馏分以剂量和时间依赖的方式降低了 ACHN 细胞的活力，而对 GP-293 细胞系没有影响。此外，总提取物导致 ACHN 细胞的形态变化比 GP-293 细胞更多。总提取物在 ACHN 细胞系中诱导凋亡的作用在 48 小时后大于在 GP-293 细胞系中。此外，在所有使用的浓度下，两种馏分的作用均低于总提取物。因此，总提取物和黑种草的正己烷和乙酸乙酯馏分对 ACHN 细胞系的细胞活力和凋亡的影响大于对 GP-293 细胞系的影响。然而，总提取物的作用高于两种馏分各自的作用。
