Department of Chemical Engineering, Indian Institute of Technology Guwahati, Guwahati 781 039, Assam, India.
Department of Chemical Engineering, Indian Institute of Technology Guwahati, Guwahati 781 039, Assam, India.
Bioresour Technol. 2016 Sep;216:1058-65. doi: 10.1016/j.biortech.2016.01.100. Epub 2016 Feb 2.
This study has addressed the matter of optimization of production of the value added product, dihydroxyacetone, from crude glycerol using immobilized cells of Gluconobacter oxydans. Statistical optimization of the fermentation medium revealed MgSO4·7H2O, (NH4)2SO4 and KH2PO4 as the significant components, in addition to small concentration of yeast extract. As per previous literature, these components augment the activity of glycerol dehydrogenase enzyme in metabolism and provide assimilable nitrogen and sulfur source for cell growth. Yeast extract not only provides essential growth factors, but also accelerates production of alcohol dehydrogenase enzyme due to amino acids present. The DHA yield from crude glycerol (20g/L) with optimized medium is 14.08g/L, which is just 12% lower than the yield for pure glycerol .This study has thus established that proper optimization of fermentation medium reduces the adverse effect of impurities in crude glycerol on fermentation process and DHA yield.
本研究采用固定化细胞氧化葡萄糖酸杆菌从粗甘油生产增值产品二羟丙酮。通过统计优化发酵培养基发现，MgSO4·7H2O、(NH4)2SO4 和 KH2PO4 除了少量酵母提取物外，也是重要的组成成分。根据以往的文献，这些成分可以提高甘油脱氢酶在新陈代谢中的活性，为细胞生长提供可同化的氮源和硫源。酵母提取物不仅提供必需的生长因子，而且由于存在氨基酸，还可以加速醇脱氢酶的产生。用优化后的培养基从粗甘油（20g/L）中得到的 DHA 产量为 14.08g/L，仅比纯甘油的产量低 12%。本研究表明，适当优化发酵培养基可以降低粗甘油中杂质对发酵过程和 DHA 产量的不利影响。
