Chattaoui M, Raya M C, Bouri M, Moral J, Perez-Rodriguez M, Trapero A, Msallem M, Rhouma A
Labaratory of Improvement and protection of olive genetic resources, Olive Tree Institute, Tunis, Tunisia.
Departamento de Agronomía (Patologia Agroforestal), Universidad de Córdoba, Córdoba, Spain.
J Appl Microbiol. 2016 May;120(5):1368-81. doi: 10.1111/jam.13096. Epub 2016 Apr 4.
To phenotypically, physiologically and molecularly characterize the causal agent of olive anthracnose in the northern Tunisia and to study its genetic variability and pathogenicity.
A total of 43 isolates were obtained from symptomatic olives collected from four regions in northern Tunisia. A range of morphological and physiological characteristics was recorded; and a phylogenetic study, based on the sequence analysis of both internal transcribed spacers and TUB2 gene regions, was performed. Of the 43 isolates, 41 were identified as Colletotrichum acutatum s.s, and only two were affiliated to Colletotrichum gloeosporioides s.s. Two more representative Spanish isolates, included for comparison, were identified as Colletotrichum godetiae. Using six inter-simple-sequence-repeat markers, homogeneity between isolates from different locations and within the same species was recorded. In pathogenicity and virulence studies, C. gloeosporioides s.s was found to be less virulent, while the Spanish C. godetiae isolate was significantly more virulent than the Tunisian C. acutatum s.s.
Olive anthracnose in the North of Tunisia is mainly caused by C. acutatum s.s species.
This is the first study of olive anthracnose in Tunisia, which combines both phenotypic and molecular approaches. Colletotrichum acutatum s.s group was recorded for the first time in the country as the causal agent of olive anthracnose.
从表型、生理和分子水平对突尼斯北部橄榄炭疽病的病原菌进行特征分析，并研究其遗传变异性和致病性。
从突尼斯北部四个地区采集的有症状橄榄中分离得到43个菌株。记录了一系列形态和生理特征；并基于内部转录间隔区和TUB2基因区域的序列分析进行了系统发育研究。在43个菌株中，41个被鉴定为尖孢炭疽菌狭义种，只有两个属于胶孢炭疽菌狭义种。另外两个用于比较的西班牙代表性菌株被鉴定为戈氏炭疽菌。使用六个简单序列重复区间标记，记录了不同地点和同一物种内菌株之间的同源性。在致病性和毒力研究中，发现胶孢炭疽菌狭义种的毒力较弱，而西班牙的戈氏炭疽菌菌株的毒力明显高于突尼斯的尖孢炭疽菌狭义种。
突尼斯北部的橄榄炭疽病主要由尖孢炭疽菌狭义种引起。
这是突尼斯首次对橄榄炭疽病进行的研究，该研究结合了表型和分子方法。尖孢炭疽菌狭义种群首次在该国被记录为橄榄炭疽病的病原菌。
