Kšonžeková Petra, Bystrický Peter, Vlčková Silvia, Pätoprstý Vladimír, Pulzová Lucia, Mudroňová Dagmar, Kubašková Terézia, Csank Tomáš, Tkáčiková Ľudmila
Department of Microbiology and Immunology, University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 041 81 Košice, Slovakia.
Institute of Chemistry, Slovak Academy of Sciences, Dúbravská Cesta 9, 845 38 Bratislava, Slovakia.
Carbohydr Polym. 2016 May 5;141:10-9. doi: 10.1016/j.carbpol.2015.12.037. Epub 2015 Dec 17.
The aim of the study was to characterize exopolysaccharides (EPS) originated from Lactobacillus reuteri strain DSM 17938 (EPS-DSM17938) and L. reuteri strain L26 Biocenol™ (EPS-L26) and evaluate their influence on adherence of enterotoxigenic Escherichia coli (ETEC) to IPEC-1 cells and proinflammatory gene expression. Both EPS were d-glucan polysaccharides with higher molecular weight (Mw), but differing in spatial conformation and elicited variable cytokine profile. EPS-DSM17938, relatively linear polysaccharide with (1→4) and (1→6) glycosidic linkages, increased IL-1β gene expression (0.1mg/mL; P<0.05), while EPS-L26, more branched polysaccharide with (1→3) and (1→6) glycosidic linkages, exerted slight but statistically significant up-regulation of NF-κB, TNF-α and IL-6 mRNA (P<0.05). The most significant finding is that preincubation of IPEC-1 cells with both EPS followed by ETEC infection inhibit ETEC adhesion on IPEC-1 cells (P<0.01) and ETEC-induced gene expression of proinflammatory cytokine IL-1β and IL-6 (P<0.01).
本研究的目的是对源自罗伊氏乳杆菌DSM 17938菌株（EPS-DSM17938）和罗伊氏乳杆菌L26 Biocenol™菌株（EPS-L26）的胞外多糖（EPS）进行表征，并评估它们对产肠毒素大肠杆菌（ETEC）黏附于IPEC-1细胞以及促炎基因表达的影响。两种EPS均为具有较高分子量（Mw）的d-葡聚糖多糖，但空间构象不同，且引发的细胞因子谱也不同。EPS-DSM17938是一种相对线性的多糖，具有（1→4）和（1→6）糖苷键，可增加IL-1β基因表达（0.1mg/mL；P<0.05），而EPS-L26是一种具有更多分支的多糖，具有（1→3）和（1→6）糖苷键，可使NF-κB、TNF-α和IL-6 mRNA有轻微但具有统计学意义的上调（P<0.05）。最显著的发现是，IPEC-1细胞先用两种EPS预孵育，然后进行ETEC感染，可抑制ETEC对IPEC-1细胞的黏附（P<0.01）以及ETEC诱导的促炎细胞因子IL-1β和IL-6的基因表达（P<0.01）。
