Harmayani Eni
Department of Animal Product Technology, Faculty of Animal Science,Universitas Gadjah Mada, Jl. Fauna 3, Kampus UGM, Bulaksumur, Yogyakarta 55281, Indonesia.
Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia.
Korean J Food Sci Anim Resour. 2015;35(6):847-58. doi: 10.5851/kosfa.2015.35.6.847. Epub 2015 Dec 31.
The study aimed to evaluate the effect of kefir combination from goat milk and soy milk on lipid profile, plasma glucose, glutathione peroxidase (GPx) activity and the improvement of pancreatic β-cell in diabetic rats. Male rats were divided into five treatments: normal control, diabetic control, goat milk kefir, combination of goat milk-soy milk kefir and soy milk kefir. All rats were induced by streptooztocin-nicotinamide (STZ-NA), except for normal control. After 35 d experiment, the rats were sampled for blood, sacrificed and sampled for pancreatic tissues. Results showed that diabetic rats fed kefir combination had higher (p<0.05) triglyceride than the rats fed goat milk or soy milk kefir. Decreasing of plasma glucose in diabetic rats fed kefir combination was higher (p<0.05) than rats fed goat millk kefir. The activity of GPx in diabetic rats fed three kinds of kefir were higher (p<0.01) than untreated diabetic rats. The average number of Langerhans and β-cells in diabetic rats fed kefir combination was the same as the normal control, but it was higher than diabetic control. It was concluded that kefir combination can be used as antidiabetic through maintaining in serum triglyceride, decreasing in plasma glucose, increasing in GPx activity and improving in pancreatic β-cells.
该研究旨在评估山羊奶和豆奶发酵乳杆菌组合对糖尿病大鼠血脂、血糖、谷胱甘肽过氧化物酶（GPx）活性以及胰腺β细胞改善情况的影响。雄性大鼠被分为五种处理组：正常对照组、糖尿病对照组、山羊奶发酵乳杆菌组、山羊奶 - 豆奶发酵乳杆菌组合组和豆奶发酵乳杆菌组。除正常对照组外，所有大鼠均通过链脲佐菌素 - 烟酰胺（STZ - NA）诱导。经过35天的实验后，对大鼠进行采血，处死后采集胰腺组织样本。结果显示，喂食发酵乳杆菌组合的糖尿病大鼠甘油三酯水平高于喂食山羊奶或豆奶发酵乳杆菌的大鼠（p<0.05）。喂食发酵乳杆菌组合的糖尿病大鼠血糖下降幅度高于喂食山羊奶发酵乳杆菌的大鼠（p<0.05）。喂食三种发酵乳杆菌的糖尿病大鼠的GPx活性高于未处理的糖尿病大鼠（p<0.01）。喂食发酵乳杆菌组合的糖尿病大鼠的朗格汉斯细胞和β细胞平均数量与正常对照组相同，但高于糖尿病对照组。研究得出结论，发酵乳杆菌组合可通过维持血清甘油三酯水平、降低血糖、提高GPx活性和改善胰腺β细胞来用作抗糖尿病物质。
