Jeong Seol-Ha, Koh Young-Hag, Kim Suk-Wha, Park Ji-Ung, Kim Hyoun-Ee, Song Juha
Department of Materials Science and Engineering, Seoul National University , Seoul 151-742, Korea.
School of Biomedical Engineering, Korea University , Seoul 136-703, Korea.
Biomacromolecules. 2016 Mar 14;17(3):841-51. doi: 10.1021/acs.biomac.5b01557. Epub 2016 Feb 15.
Hyaluronic acid (HAc) hydrogel exhibits excellent biocompatibility, but it has limited biomedical application due to its poor biomechanical properties as well as too-fast enzymatic degradation. In this study, we have developed an in situ precipitation process for the fabrication of a HAc-calcium phosphate nanocomposite hydrogel, after the formation of the glycidyl methacrylate-conjugated HAc (GMHA) hydrogels via photo-cross-linking, to improve the mechanical and biological properties under physiological conditions. In particular, our process facilitates the rapid incorporation of calcium phosphate (CaP) nanoparticles of uniform size and with minimal agglomeration into a polymer matrix, homogeneously. Compared with pure HAc, the nanocomposite hydrogels exhibit improved mechanical behavior. Specifically, the shear modulus is improved by a factor of 4. The biostability of the nanocomposite hydrogel was also significantly improved compared with that of pure HAc hydrogels under both in vitro and in vivo conditions.
透明质酸（HAc）水凝胶具有优异的生物相容性，但由于其生物力学性能较差以及酶促降解过快，其生物医学应用受到限制。在本研究中，我们开发了一种原位沉淀工艺，用于制备HAc-磷酸钙纳米复合水凝胶。该工艺是在通过光交联形成甲基丙烯酸缩水甘油酯共轭HAc（GMHA）水凝胶之后进行的，目的是在生理条件下改善其机械性能和生物学性能。特别是，我们的工艺有助于将尺寸均匀且团聚最小的磷酸钙（CaP）纳米颗粒快速均匀地掺入聚合物基质中。与纯HAc相比，纳米复合水凝胶表现出改善的机械性能。具体而言，剪切模量提高了4倍。在体外和体内条件下，纳米复合水凝胶的生物稳定性也比纯HAc水凝胶有显著提高。
