Patyshakuliyeva Aleksandrina, Arentshorst Mark, Allijn Iris E, Ram Arthur F J, de Vries Ronald P, Gelber Isabelle Benoit
Fungal Molecular Physiology, CBS-KNAW Fungal Biodiversity Centre, Utrecht University, Utrecht, The Netherlands.
Molecular Microbiology and Biotechnology, Leiden University, Leiden, The Netherlands.
Biotechnol Lett. 2016 Jun;38(6):969-74. doi: 10.1007/s10529-016-2060-0. Epub 2016 Feb 15.
To evaluate the potential of adaptive evolution as a tool in generating strains with an improved production of plant biomass degrading enzymes.
An Aspergillus niger cellulase mutant was obtained by adaptive evolution. Physiological properties of this mutant revealed a five times higher cellulose production than the parental strain. Transcriptomic analysis revealed that the expression of noxR, encoding the regulatory subunit of the NADPH oxidase complex, was reduced in the mutant compared to the parental strain. Subsequent analysis of a noxR knockout strain showed the same phenotypic effect as observed for the evolution mutant, confirming the role of NoxR in cellulose degradation.
Adaptive evolution is an efficient approach to modify a strain and activate genes involved in polysaccharide degradation.
评估适应性进化作为一种工具在产生植物生物质降解酶产量提高的菌株方面的潜力。
通过适应性进化获得了一株黑曲霉纤维素酶突变体。该突变体的生理特性显示其纤维素产量比亲本菌株高五倍。转录组分析表明,与亲本菌株相比,该突变体中编码NADPH氧化酶复合体调节亚基的noxR的表达降低。随后对noxR基因敲除菌株的分析显示出与进化突变体相同的表型效应,证实了NoxR在纤维素降解中的作用。
适应性进化是修饰菌株并激活参与多糖降解的基因的有效方法。