Corradi Elisabetta, De Mieri Maria, Cadisch Larissa, Abbet Christian, Hamburger Matthias, Potterat Olivier
University of Basel, Division of Pharmaceutical Biology, Klingelbergstrasse 50, CH-4056 Basel, (phone: +41-61-2671534; fax: +41-61-2671474).
Chem Biodivers. 2016 Feb;13(2):188-97. doi: 10.1002/cbdv.201500353.
Thirteen acylated flavonoid glycosides, 1-13, including eleven new congeners, 3-13, were isolated from the aerial parts of Pritzelago alpina (Brassicaceae) by a combination of column chromatography on Sephadex LH-20, and preparative and semi-preparative HPLC. The structures were established by extensive NMR and MS experiments in combination with acid hydrolysis and sugar analysis by GC/MS. The new compounds were shown to be kaempferol and quercetin glycosides acylated for most of them by a branched short chain fatty acid or a hydroxycinnamic acid residue on the sugar portion. As shown by a HPLC-DAD analysis of a MeOH extract, these compounds are the main phenolic constituents in the aerial parts of the plant.
通过葡聚糖凝胶LH - 20柱色谱、制备型和半制备型高效液相色谱相结合的方法，从高山锥果芥（十字花科）地上部分分离出13种酰化黄酮苷（1 - 13），其中包括11个新同系物（3 - 13）。通过广泛的核磁共振（NMR）和质谱（MS）实验，结合酸水解和气相色谱/质谱（GC/MS）糖分析确定了其结构。新化合物显示为山奈酚和槲皮素苷，其中大多数在糖部分被支链短链脂肪酸或羟基肉桂酸残基酰化。通过甲醇提取物的高效液相色谱 - 二极管阵列检测（HPLC - DAD）分析表明，这些化合物是该植物地上部分的主要酚类成分。
