Cluster of Excellence Center for Advancing Electronics Dresden (cfaed), TU Dresden , 01062 Dresden, Germany.
Nano Lett. 2016 Mar 9;16(3):2108-13. doi: 10.1021/acs.nanolett.6b00381. Epub 2016 Feb 23.
DNA nanotechnology offers unique control over matter on the nanoscale. Here, we extend the DNA origami method to cover a range of wireframe truss structures composed of equilateral triangles, which use less material per volume than standard multiple-helix bundles. From a flat truss design, we folded tetrahedral, octahedral, or irregular dodecahedral trusses by exchanging few connector strands. Other than standard origami designs, the trusses can be folded in low-salt buffers that make them compatible with cell culture buffers. The structures also have defined cavities that may in the future be used to precisely position functional elements such as metallic nanoparticles or enzymes. Our graph routing program and a simple design pipeline will enable other laboratories to make use of this valuable and potent new construction principle for DNA-based nanoengineering.
DNA 纳米技术为纳米尺度的物质提供了独特的控制手段。在这里，我们扩展了 DNA 折纸方法，涵盖了一系列由等边三角形组成的网架桁架结构，这些结构每单位体积使用的材料比标准的多螺旋束少。从一个平面桁架设计开始，我们通过交换少量连接链来折叠四面体、八面体或不规则的十二面体桁架。除了标准的折纸设计，这些桁架还可以在低盐缓冲液中折叠，使其与细胞培养缓冲液兼容。这些结构还具有确定的腔，将来可能用于精确定位功能元件，如金属纳米粒子或酶。我们的图路由程序和简单的设计流程将使其他实验室能够利用这种有价值的、强大的基于 DNA 的纳米工程新构建原则。
