一种基于用二氧化硅球将漆酶固定在多壁碳纳米管掺杂的丝网印刷电极上的一次性生物传感器。
A disposable biosensor based on immobilization of laccase with silica spheres on the MWCNTs-doped screen-printed electrode.
作者信息
Li Yuanting, Zhang Li, Li Meng, Pan Zhigang, Li Dawei
机构信息
Key Laboratory for Advanced Materials & Department of Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, P R China.
出版信息
Chem Cent J. 2012 Sep 17;6(1):103. doi: 10.1186/1752-153X-6-103.
BACKGROUND
Biosensors have attracted increasing attention as reliable analytical instruments in in situ monitoring of public health and environmental pollution. For enzyme-based biosensors, the stabilization of enzymatic activity on the biological recognition element is of great importance. It is generally acknowledged that an effective immobilization technique is a key step to achieve the construction quality of biosensors.
RESULTS
A novel disposable biosensor was constructed by immobilizing laccase (Lac) with silica spheres on the surface of multi-walled carbon nanotubes (MWCNTs)-doped screen-printed electrode (SPE). Then, it was characterized in morphology and electrochemical properties by scanning electron microscopy (SEM) and cyclic voltammetry (CV). The characterization results indicated that a high loading of Lac and a good electrocatalytic activity could be obtained, attributing to the porous structure, large specific area and good biocompatibility of silica spheres and MWCNTs. Furthermore, the electrochemical sensing properties of the constructed biosensor were investigated by choosing dopamine (DA) as the typical model of phenolic compounds. It was shown that the biosensor displays a good linearity in the range from 1.3 to 85.5 μM with a detection limit of 0.42 μM (S/N = 3), and the Michaelis-Menten constant (Kmapp) was calculated to be 3.78 μM.
CONCLUSION
The immobilization of Lac was successfully achieved with silica spheres to construct a disposable biosensor on the MWCNTs-doped SPE (MWCNTs/SPE). This biosensor could determine DA based on a non-oxidative mechanism in a rapid, selective and sensitive way. Besides, the developed biosensor could retain high enzymatic activity and possess good stability without cross-linking reagents. The proposed immobilization approach and the constructed biosensor offer a great potential for the fabrication of the enzyme-based biosensors and the analysis of phenolic compounds.
背景
生物传感器作为公共卫生和环境污染现场监测的可靠分析仪器，已引起越来越多的关注。对于基于酶的生物传感器，生物识别元件上酶活性的稳定至关重要。人们普遍认为，有效的固定化技术是实现生物传感器构建质量的关键步骤。
结果
通过将漆酶（Lac）与二氧化硅球固定在多壁碳纳米管（MWCNTs）掺杂的丝网印刷电极（SPE）表面，构建了一种新型一次性生物传感器。然后，通过扫描电子显微镜（SEM）和循环伏安法（CV）对其形态和电化学性质进行了表征。表征结果表明，由于二氧化硅球和MWCNTs的多孔结构、大比表面积和良好的生物相容性，可以获得高负载量的Lac和良好的电催化活性。此外，选择多巴胺（DA）作为酚类化合物的典型模型，研究了构建的生物传感器的电化学传感性能。结果表明，该生物传感器在1.3至85.5 μM范围内显示出良好的线性，检测限为0.42 μM（S/N = 3），米氏常数（Kmapp）计算为3.78 μM。
结论
成功地用二氧化硅球实现了Lac的固定化，在MWCNTs掺杂的SPE（MWCNTs/SPE）上构建了一次性生物传感器。该生物传感器可以基于非氧化机制快速、选择性和灵敏地测定DA。此外，所开发的生物传感器可以保持高酶活性并具有良好的稳定性，无需交联试剂。所提出的固定化方法和构建的生物传感器为基于酶的生物传感器的制造和酚类化合物的分析提供了巨大的潜力。
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