Yan Zheng-Fei, Lin Pei, Chu Xiao, Kook MooChang, Li Chang-Tian, Yi Tae-Hoo
Department of Oriental Medicine Biotechnology, College of Life Science, Kyung Hee University Global Campus, 1732 Deokyoungdaero, Giheung-gu, Yongin-si, Gyeonggi-do, 446-701, Republic of Korea.
Engineering Research Center of Edible and Medicinal Fungi, Ministry of Education, Jilin Agricultural University, Changchun, 130118, People's Republic of China.
Arch Microbiol. 2016 Jul;198(5):423-7. doi: 10.1007/s00203-016-1196-6. Epub 2016 Feb 18.
A Gram-positive, aerobic, and non-motile, rod-shaped actinomycete strain, designated YIM Y47(T), was isolated from soils collected from Turpan desert, China, and subjected to a polyphasic taxonomic study. Phylogenetic analysis indicated that strain YIM Y47(T) belonged to the genus Aeromicrobium. YIM Y47(T) shared highest 16S rRNA gene sequence similarities with Aeromicrobium massiliense JC14(T) (96.47 %). Growth occurs at 20-45 °C (optimum at 30 °C), pH 6.0-8.0 (optimum at pH 7.0), and salinities of 0-7.0 % NaCl (optimum at 4.0 %). The strain YIM Y47(T) exhibits chemotaxonomic features with menaquinone-7 (MK-7) as the predominant quinone, C16:0, C18:1 ω9c and 10-methyl C18:0 (>10 %) as major fatty acids. The cell-wall peptidoglycan of strain YIM Y47(T) contained LL-diaminopimelic acid as the diagnostic diamino acid. The polar lipids were found to consist of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, and unknown phospholipids. The G+C content of the genomic DNA of strain YIM Y47(T) was found to be 44.7 mol%. On the basis of phylogenetic analyses and phenotypic data, it is proposed that strain YIM Y47(T) should be classified as representing a novel species of the genus Aeromicrobium, with the name Aeromicrobium halotolerans sp. nov. The type strain is YIM Y47(T) (=KCTC 39113(T)=CGMCC 1.15063(T)=DSM 29939(T)=JCM 30627(T)).
从中国吐鲁番沙漠采集的土壤中分离出一株革兰氏阳性、需氧、无运动性的杆状放线菌，命名为YIM Y47(T)，并对其进行了多相分类研究。系统发育分析表明，菌株YIM Y47(T)属于嗜气微菌属。YIM Y47(T)与马赛嗜气微菌JC14(T)的16S rRNA基因序列相似性最高（96.47%）。该菌在20-45°C（最适温度为30°C）、pH 6.0-8.0（最适pH为7.0）和0-7.0% NaCl盐度（最适盐度为4.0%）条件下生长。菌株YIM Y47(T)呈现出化学分类学特征，以甲基萘醌-7（MK-7）作为主要醌类，C16:0、C18:1 ω9c和10-甲基C18:0（>10%）作为主要脂肪酸。菌株YIM Y47(T)的细胞壁肽聚糖含有LL-二氨基庚二酸作为诊断性二氨基酸。发现极性脂质由双磷脂酰甘油、磷脂酰甘油、磷脂酰肌醇和未知磷脂组成。菌株YIM Y47(T)基因组DNA的G+C含量为44.7 mol%。基于系统发育分析和表型数据，建议将菌株YIM Y47(T)分类为嗜气微菌属的一个新物种，命名为嗜盐嗜气微菌（Aeromicrobium halotolerans sp. nov.）。模式菌株为YIM Y47(T)（=KCTC 39113(T)=CGMCC 1.15063(T)=DSM 29939(T)=JCM 30627(T)）。
