Department of Soil and Environmental Sciences, College of Agriculture and Natural Resources, National Chung Hsing University, Taichung City, Taiwan, ROC.
Innovation and Development Center of Sustainable Agriculture, National Chung Hsing University, Taichung, Taiwan, ROC.
Int J Syst Evol Microbiol. 2021 Feb;71(2). doi: 10.1099/ijsem.0.004616.
A polyphasic taxonomic approach was used to characterize a Gram-stain-positive bacterium, designated strain CC-CFT486, isolated from soil sampled in a maize field in Taiwan. Cells of strain CC-CFT486 were short rods, motile with polar flagella, catalase-positive and oxidase-positive. Optimal growth occurred at 30 °С, pH 8 and 1 % NaCl. Phylogenetic analyses based on 16S rRNA genes revealed a distinct taxonomic position attained by strain CC-CFT486 associated with (97.0 % sequence identity), (97.0 %), (96.8 %) and (96.8 %), and lower sequence similarity values to other species. Average nucleotide identity (ANI) values were 70.6-77.8 % (=11) compared within the type strains of the genus . Strain CC-CFT486 contained C, C, C 8c and C 9c as the predominant fatty acids. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, two unidentified aminophospholipids and three unknown phospholipids. The cell wall peptidoglycan of strains CC-CFT486 contained ll-diaminopimelic acid (ll-DAP) and the major polyamine was spermidine. The DNA G+C content was 70.6 mol% and the predominant quinone was menaquinone 9 (MK-9). Based on its distinct phylogenetic, phenotypic and chemotaxonomic traits together with results of comparative 16S rRNA gene sequence and ANI analyses, strain CC-CFT486 is proposed to represent a novel species, for which the name sp. nov. (type strain CC-CFT486=BCRC 81217=JCM 33499).
采用多相分类法对一株革兰氏阳性菌进行了表征，该菌命名为 CC-CFT486 菌株，分离自台湾玉米地土壤。CC-CFT486 菌株的细胞为短杆状，有极生鞭毛，运动，接触酶和氧化酶阳性。最适生长温度为 30°C，pH8 和 1%NaCl。基于 16S rRNA 基因的系统发育分析表明，CC-CFT486 菌株与 （97.0%序列同一性）、 （97.0%）、 （96.8%）和 （96.8%）密切相关，与其他种的序列相似性较低。与属内的模式菌株相比，CC-CFT486 菌株的平均核苷酸同一性（ANI）值为 70.6-77.8%（=11）。CC-CFT486 菌株含有 C 16:0、C 18:1ω7c 和 C 9c 作为主要脂肪酸。极性脂谱由双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甘油、磷脂酰肌醇、两种未鉴定的氨基磷脂和三种未知磷脂组成。CC-CFT486 菌株的细胞壁肽聚糖含有 ll-二氨基庚二酸（ll-DAP），主要聚胺为亚精胺。DNA G+C 含量为 70.6mol%，主要醌为menaquinone 9（MK-9）。基于其独特的系统发育、表型和化学分类特征，以及 16S rRNA 基因序列和 ANI 分析的比较结果，CC-CFT486 菌株代表了一个新的 种，命名为 sp. nov.（模式菌株 CC-CFT486=BCRC 81217=JCM 33499）。
