State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology , Beijing 100029, China.
Anal Chem. 2016 Mar 1;88(5):2659-65. doi: 10.1021/acs.analchem.5b03827. Epub 2016 Feb 19.
There is currently great interest in developing chemiluminescence (CL) probes that can selectively detect peroxynitrite (ONOO(-)) in living cells. In comparison with other reactive oxygen species (ROS), ONOO(-) can spontaneously decompose into a series of radicals. Notably, the interaction of quantum dots (QDs) with oxidizing/reducing ROS radicals can generate a strong CL emission by electron-transfer annihilation. Herein, we report a novel CL probe that affords the ability to distinguish ONOO(-) from other ROS in living cells. ONOO(-) can activate luminescence of QDs in the absence of excitation source, effectively avoiding background noise and scattering of light from biological matrixes produced by in situ excitation; however, there is no response to other ROS including (1)O2, H2O2, (•)OH, O2(•-), and ClO(-). The outstanding selectivity of the present CL probe leads us to detect the exogenous release of ONOO(-) from 3-morpholinosydnonimine (SIN-1) in living cells. These results suggest that this present probe-based CL provides a promising platform for highly selective and sensitive detection of ONOO(-) in biological systems.
目前人们对开发能够选择性检测活细胞中过氧亚硝酸盐 (ONOO(-)) 的化学发光 (CL) 探针非常感兴趣。与其他活性氧 (ROS) 相比，ONOO(-) 可以自发分解成一系列自由基。值得注意的是，量子点 (QDs) 与氧化/还原 ROS 自由基的相互作用可以通过电子转移湮灭产生强 CL 发射。在此，我们报告了一种新型 CL 探针，它能够区分活细胞中的 ONOO(-) 和其他 ROS。在没有激发源的情况下，ONOO(-) 可以激活 QDs 的发光，有效避免了由原位激发产生的生物基质的背景噪声和光散射；然而，它对其他 ROS （包括 (1)O2、H2O2、(•)OH、O2(•-) 和 ClO(-)）没有响应。本 CL 探针的出色选择性使我们能够检测到 3-吗啉代-sydnonimine (SIN-1) 在活细胞中外源释放的 ONOO(-)。这些结果表明，这种基于探针的 CL 为在生物系统中高度选择性和灵敏地检测 ONOO(-) 提供了一个有前途的平台。
