Antony Jesu Prabhu P, Geurden Inge, Fontagné-Dicharry Stéphanie, Veron Vincent, Larroquet Laurence, Mariojouls Catherine, Schrama Johan W, Kaushik Sadasivam J
Institut National de la Recherche Agronomique (INRA), UR1067, Nutrition, Metabolism and Aquaculture (NuMeA), Aquapôle INRA, 64310, Saint-Pée-sur-Nivelle, France.
AgroParisTech, Paris Institute of Technology for Life, Food and Environmental Sciences, 16 rue Claude Bernard, 75231, Paris, Cedex 5, France.
PLoS One. 2016 Feb 19;11(2):e0149378. doi: 10.1371/journal.pone.0149378. eCollection 2016.
Responses in micro-mineral metabolism to changes in dietary ingredient composition and inclusion of a micro-mineral premix (Fe, Cu, Mn, Zn and Se) were studied in rainbow trout. In a 2 x 2 factorial design, triplicate groups of rainbow trout (initial weight: 20 g) were fed over 12 weeks at 17°C a fishmeal-based diet (M) or a plant-ingredient based diet (V), with or without inclusion of a mineral premix. Trout fed the V vs. M diet had lower feed intake, growth, hepato-somatic index, apparent availability coefficient (AAC) of Fe, Cu, Mn and Zn and also lower whole body Se and Zn concentration, whereas whole body Fe and Cu and plasma Fe concentrations were higher. Feeding the V diet increased intestinal ferric reductase activity; at transcriptional level, hepatic hepcidin expression was down-regulated and ferroportin 1 was up-regulated. Transcription of intestinal Cu-transporting ATPases and hepatic copper transporter1 were higher in V0 compared to other groups. Among the hepatic metalo-enzyme activities assayed, only Se-dependent glutathione peroxidase was affected, being lower in V fed fish. Premix inclusion reduced the AAC of Fe, Cu and Zn; increased the whole body concentration of all micro- minerals; up-regulated hepatic hepcidin and down-regulated intestinal ferroportin 1 transcription; and reduced the transcription of Cu-transporting ATPases in the intestine. Overall, the regulation of micro-mineral metabolism in rainbow trout, especially Fe and Cu, was affected both by a change in ingredient composition and micro-mineral premix inclusion.
研究了虹鳟鱼对日粮成分变化以及添加微量矿物质预混料（铁、铜、锰、锌和硒）的微量矿物质代谢反应。采用2×2析因设计，将虹鳟鱼（初始体重：20克）分成三组，每组设三个重复，在17℃下，投喂12周，分别喂以鱼粉型日粮（M）或植物成分型日粮（V），添加或不添加矿物质预混料。与喂M日粮的虹鳟鱼相比，喂V日粮的虹鳟鱼采食量、生长速度、肝体指数、铁、铜、锰和锌的表观消化率系数（AAC）较低，同时鱼体硒和锌浓度也较低，而鱼体铁和铜以及血浆铁浓度较高。投喂V日粮可提高肠道铁还原酶活性；在转录水平上，肝脏铁调素表达下调，铁转运蛋白1上调。与其他组相比，V0组肠道铜转运ATP酶和肝脏铜转运蛋白1的转录水平更高。在所测定的肝脏金属酶活性中，只有硒依赖性谷胱甘肽过氧化物酶受到影响，喂V日粮的鱼中该酶活性较低。添加预混料降低了铁、铜和锌的AAC；提高了所有微量矿物质的鱼体浓度；上调了肝脏铁调素，下调了肠道铁转运蛋白1的转录；并降低了肠道铜转运ATP酶的转录。总体而言，日粮成分变化和微量矿物质预混料添加均影响虹鳟鱼微量矿物质代谢的调节，尤其是铁和铜的调节。
