Xu Jing, Gao Chao, Zhang Fang, Ma Xiaofeng, Peng Xiaolin, Zhang Rongxin, Kong Dexin, Simard Alain R, Hao Junwei
Department of Neurology, Tianjin Neurological Institute, Tianjin Medical University General Hospital, Tianjin 300052, China.
Laboratory of Immunology and Inflammation, Research Center of Basic Medical Science, Tianjin Medical University, Tianjin 300070, China.
Sci Rep. 2016 Feb 22;6:21819. doi: 10.1038/srep21819.
The aim of our present study was to determine whether message RNAs (mRNAs) and long noncoding RNAs (lncRNAs) are expressed differentially in patients with Guillain-Barré syndrome (GBS) compared with healthy controls. The mRNA and lncRNA profiles of GBS patients and healthy controls were generated by using microarray analysis. From microarray analysis, we listed 310 mRNAs and 114 lncRNAs with the mRMR software classed into two sample groups, GBS patients and healthy controls. KEGG mapping demonstrated that the top seven signal pathways may play important roles in GBS development. Several GO terms, such as cytosol, cellular macromolecular complex assembly, cell cycle, ligase activity, protein catabolic process, etc., were enriched in gene lists, suggesting a potential correlation with GBS development. Co-expression network analysis indicated that 113 lncRNAs and 303 mRNAs were included in the co-expression network. Our present study showed that these differentially expressed mRNAs and lncRNAs may play important roles in GBS development, which provides basic information for defining the mechanism(s) that promote GBS.
我们当前研究的目的是确定与健康对照相比，吉兰-巴雷综合征（GBS）患者的信使核糖核酸（mRNA）和长链非编码核糖核酸（lncRNA）表达是否存在差异。通过使用微阵列分析生成GBS患者和健康对照的mRNA和lncRNA谱。通过微阵列分析，我们用mRMR软件列出了310个mRNA和114个lncRNA，并将其分为GBS患者和健康对照两个样本组。KEGG映射表明，前七条信号通路可能在GBS发展中起重要作用。几个基因本体论（GO）术语，如胞质溶胶、细胞大分子复合物组装、细胞周期、连接酶活性、蛋白质分解代谢过程等，在基因列表中富集，表明与GBS发展可能存在相关性。共表达网络分析表明，共表达网络中包含113个lncRNA和303个mRNA。我们目前的研究表明，这些差异表达的mRNA和lncRNA可能在GBS发展中起重要作用，这为确定促进GBS的机制提供了基础信息。
