Lee Min Jung, Jang Minhee, Bae Chun-Sik, Park Kyoung-Sun, Kim Hak-Jae, Lee Sanghyun, Lee Sang Won, Kim Young Ock, Cho Ik-Hyun
1 Department of Convergence Medical Science, College of Korean Medicine, Kyung Hee University , Seoul, Republic of Korea.
2 Brain Korea 21 Plus Program, Kyung Hee University , Seoul, Republic of Korea.
Rejuvenation Res. 2016 Dec;19(6):456-466. doi: 10.1089/rej.2015.1787. Epub 2016 Mar 31.
A traditional herbal prescription Kyung-Ok-Ko (KOK), composed of Rehmannia glutinosa Liboschitz var. purpurae, Lycium chinense, Aquilaria agallocha, Poria cocos, Panax ginseng, and honey, has been widely used in Oriental medicine as an invigorant for age-related diseases, such as amnesia and stroke. However, the beneficial value of KOK on uterine dysfunction related to hyperandrogenism is largely unknown. We investigated the effect of KOK (2.0 g/kg/day, per os) on endometrial abnormalities in a dehydroepiandrosterone (DHEA, subcutaneous)-induced polycystic ovary syndrome (PCOS) rat model. Preadministration of KOK significantly (p<0.05) decreased the elevated body weight, uterus weight, and endometrial thickness by PCOS induction, corresponding to reduced apoptosis and the infiltration of immune cells (CD4 T cells, CD8 T cells, and macrophages) in the endometrium. These results were associated with reduced mRNA expression of interleukin (IL)-1β, IL-6, IL-8, and matrix metalloproteinase-3 and increased mRNA expression of IGF-β1, transforming growth factor (TGF)-β, TGF-β1, and vascular endothelial growth factor in the uterus after DHEA injection. These multiple effects of KOK may synergistically prevent the development of endometrial abnormalities in DHEA-induced hyperandrogenism via anti-inflammatory action, indicating that KOK has preventive and therapeutic potential for suppressing PCOS.
一种传统草药配方京玉膏（KOK），由紫地黄、枸杞、沉香、茯苓、人参和蜂蜜组成，在东方医学中已被广泛用作治疗与年龄相关疾病（如失忆和中风）的滋补剂。然而，KOK对与高雄激素血症相关的子宫功能障碍的有益价值在很大程度上尚不清楚。我们研究了KOK（2.0克/千克/天，口服）对脱氢表雄酮（DHEA，皮下注射）诱导的多囊卵巢综合征（PCOS）大鼠模型子宫内膜异常的影响。预先给予KOK可显著（p<0.05）降低因PCOS诱导而升高的体重、子宫重量和子宫内膜厚度，这与子宫内膜中细胞凋亡减少以及免疫细胞（CD4 T细胞、CD8 T细胞和巨噬细胞）浸润减少相对应。这些结果与注射DHEA后子宫中白细胞介素（IL）-1β、IL-6、IL-8和基质金属蛋白酶-3的mRNA表达降低以及IGF-β1、转化生长因子（TGF）-β、TGF-β1和血管内皮生长因子的mRNA表达增加有关。KOK的这些多种作用可能通过抗炎作用协同预防DHEA诱导的高雄激素血症中子宫内膜异常的发展，表明KOK具有抑制PCOS的预防和治疗潜力。
