Kim Yong-Goo, Komakech Richard, Jeong Dae Hui, Park Yun Mi, Lee Tae Kyoung, Kim Ki Hyun, Lee A Yeong, Moon Byeong Cheol, Kang Youngmin
Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine (KIOM), 111 Geonjae-ro, Naju-si, Jeollanam-do 58245, Korea.
Korean Medicine Life Science Major, University of Science & Technology (UST), Campus of KIOM, Daejeon 34054, Korea.
Plants (Basel). 2020 Mar 3;9(3):317. doi: 10.3390/plants9030317.
(Gaertn.) DC is a perennial plant belonging to the family Scropulariidae. The root of is used in oriental medicine and mainly grown using rootstock rather than seed cultivation, which gives rise to several problems including root rot, and results in a low productivity and poor quality. To solve the challenges involved in seed cultivation, our team previously used the formative features and genetic analysis of to determine the optimal in vitro tissue culture conditions for producing sterile culture seedlings and rootstocks of . The aim of the present study was to identify differences between standard rootstock seedlings (SR), culture rootstock seedlings (CR), and culture seedlings (CS) under field conditions. The reproductive characteristics of the aerial part were more robust while the area and length of leaves were smaller for SR than those for CR and CS. The characteristic that differed the most in SR was flowering, which did not occur in CR and CS. In addition, the fresh and dry weights of the subterranean parts of CR and CS were two-fold greater than those of SR. Fourier transform near-infrared (FT-NIR) analysis showed only slight differences between the chemical constituents of SR and its culture products, which was confirmed by measuring the content of catalpol, an indexing substance. Catalpol had a reduced content in the culture products compared to SR. However, this difference was not significant. Our findings will be useful for the identification of the best seedling type of to enable its mass production.
（Gaertn.）DC是一种隶属于玄参科的多年生植物。其根在东方医学中被使用，主要通过根茎繁殖而非种子培育，这引发了包括根腐病在内的诸多问题，导致产量低且品质差。为解决种子培育中涉及的挑战，我们团队此前利用其形态特征和遗传分析来确定生产无菌培养幼苗和根茎的最佳体外组织培养条件。本研究的目的是在田间条件下确定标准根茎幼苗（SR）、培养根茎幼苗（CR）和培养幼苗（CS）之间的差异。地上部分的生殖特征对于SR而言更为强健，而SR的叶片面积和长度小于CR和CS。SR中差异最大的特征是开花，CR和CS均未开花。此外，CR和CS地下部分的鲜重和干重是SR的两倍。傅里叶变换近红外（FT-NIR）分析表明，SR及其培养产物的化学成分之间仅有细微差异，这通过测量梓醇（一种指标性物质）的含量得到证实。与SR相比，培养产物中梓醇的含量有所降低。然而，这种差异并不显著。我们的研究结果将有助于确定最佳的幼苗类型以实现其大规模生产。
