Jiang Cheng, Alam Muhammad Tanzirul, Parker Stephen G, Darwish Nadim, Gooding J Justin
School of Chemistry, Australian Centre for NanoMedicine and ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, The University of New South Wales , Sydney, NSW 2052, Australia.
Langmuir. 2016 Mar 15;32(10):2509-17. doi: 10.1021/acs.langmuir.5b04550. Epub 2016 Mar 3.
Controlling the composition of an interface is very important in tuning the chemical and physical properties of a surface in many applications including biosensors, biomaterials, and chemical catalysis. Frequently, this requires one molecular component to a minor component in a mixed layer. Such subtle control of composition has been difficult to achieve using aryldiazonium salts. Herein, aryldiazonium salts of carboxyphenyl (CP) and phenylphosphorylcholine (PPC), generated in situ from their corresponding anilines, are electrografted to form molecular platform that are available for further functionalization. These two components are chosen because CP provides a convenient functionality for further coupling of biorecognition species while PPC offers resistance to nonspecific adsorption of proteins to the surface. Mixed layers of CP and PPC were prepared by grafting them either simultaneously or consecutively. The latter strategy allows an interface to be developed in a controlled way where one component is at levels of less than 1% of the total layer.
在包括生物传感器、生物材料和化学催化在内的许多应用中，控制界面的组成对于调节表面的化学和物理性质非常重要。通常，这需要一种分子组分成为混合层中的次要组分。使用芳基重氮盐很难实现这种对组成的精细控制。在此，羧基苯基（CP）和苯基磷酰胆碱（PPC）的芳基重氮盐由它们相应的苯胺原位生成，通过电接枝形成可用于进一步功能化的分子平台。选择这两种组分是因为CP为生物识别物种的进一步偶联提供了便利的功能，而PPC则能抵抗蛋白质在表面的非特异性吸附。CP和PPC的混合层通过同时或连续接枝来制备。后一种策略允许以可控的方式形成界面，其中一种组分的含量低于总层的1%。
